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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse Peroxiredoxin 5
Met1-Leu210
Accession # P99029

Specificity

Detects human, mouse and rat Peroxiredoxin 5 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Peroxiredoxin 5

Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54‑63, and a peroxisome targeting motif at aa 208‑210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17 kDa in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83‑102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively.

Alternate Names

ACR1, AOEB166, AOPP, PMP20, PRDX5, PRXV

Entrez Gene IDs

25824 (Human); 54683 (Mouse); 113898 (Rat)

Gene Symbol

PRDX5

UniProt

Additional Peroxiredoxin 5 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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