Human/Mouse/Rat Peroxiredoxin 2 Antibody

Name: Human/Mouse/Rat Peroxiredoxin 2 Antibody
Brand: R&D Systems
SKU: AF3489
Price: 134 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF3489

Citations (4)

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human Peroxiredoxin 2 Met1-Asn198 Accession # P32119
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	The antibody detects endogenous human, mouse and rat Peroxiredoxin 2 in in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Peroxiredoxin 1 and rhPeroxiredoxin 4 is observed, approximately 10% cross-reactivity with rhPeroxiredoxin 3 is observed, and less than 1% cross‑reactivity with rhPeroxiredoxin 5 and rhPeroxiredoxin 6 is observed.

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Applications for Human/Mouse/Rat Peroxiredoxin 2 Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Peroxiredoxin 2 Antibody

	Western Blot Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
	Simple Western Detection of Human Peroxiredoxin 2 by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Peroxiredoxin 2 at approximately 27 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Peroxiredoxin 2

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Item	Value
Entrez Gene IDs	7001 (Human); 21672 (Mouse); 29338 (Rat)
Alternate Names	EC 1.11.1, MGC4104, NKEF-B, NKEFBNKEF-B, Natural killer cell-enhancing factor B, PRDX2, PRPTDPX1, PRX2, PRXII, Peroxiredoxin 2, TDPX1, TPX1, TSA, TSAEC 1.11.1.15, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, natural killer-enhancing factor B, peroxiredoxin-2, thiol-specific antioxidant 1, torin

Citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

H Alshaabi et al. (2021), Miro1-mediated mitochondrial positioning supports subcellular redox status Redox Biology, 38(0):101818.
PMID: 33341544
Species: Human
Sample Types: Whole Cells
Applications: Western Blot

KT Stein et al. (2020), Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells PLoS Comput. Biol., 16(9):e1008202.
PMID: 32925922
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

AG Barata et al. (2019), A role for peroxiredoxins in H2O2- and MEKK-dependent activation of the p38 signaling pathway Redox Biol, 28(0):101340.
PMID: 31629169
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sobotta M et al. (2015), Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling. Nat Chem Biol, 11(1):64-70.
PMID: 25402766

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Human/Mouse/Rat Peroxiredoxin 2 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat Peroxiredoxin 2 Antibody

Scientific Data Examples for Human/Mouse/Rat Peroxiredoxin 2 Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

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Background: Peroxiredoxin 2

Citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody (4)

Product Documents for Human/Mouse/Rat Peroxiredoxin 2 Antibody

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