Human/Mouse/Rat Peroxiredoxin 2 Antibody

Name: Human/Mouse/Rat Peroxiredoxin 2 Antibody
Brand: R&D Systems
SKU: MAB3489
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3489

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(3)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human Peroxiredoxin 2 Met1-Asn198 Accession # P32119
Applications:	Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 477719
Specificity:	Detects human, mouse, and rat Peroxiredoxin 2 in Western blots. In Western blots, approximately 25%-40% cross-reactivity with recombinant human (rh) Peroxiredoxin 1 and 4 is observed, and less than 10% cross-reactivity with rhPeroxiredoxin 3, 5, and 6 is observed. In direct ELISAs, approximately 15%-25% cross-reactivity with rhPeroxiredoxin 1 and 4 is observed, and less than 5% cross-reactivity with rhPeroxiredoxin 3, 5, or 6 is observed.

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Applications for Human/Mouse/Rat Peroxiredoxin 2 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Peroxiredoxin 2 Antibody

	Western Blot Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Peroxiredoxin 2 Monoclonal Antibody (Catalog # MAB3489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Peroxiredoxin 2

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Item	Value
Entrez Gene IDs	7001 (Human); 21672 (Mouse); 29338 (Rat)
Alternate Names	EC 1.11.1, MGC4104, NKEF-B, NKEFBNKEF-B, Natural killer cell-enhancing factor B, PRDX2, PRPTDPX1, PRX2, PRXII, Peroxiredoxin 2, TDPX1, TPX1, TSA, TSAEC 1.11.1.15, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, natural killer-enhancing factor B, peroxiredoxin-2, thiol-specific antioxidant 1, torin

Citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Van Brussel et al. (2012), Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress. PLoS ONE, 7(8):e43357.
PMID: 22916248

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Customer Reviews for Human/Mouse/Rat Peroxiredoxin 2 Antibody (3)

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Human/Mouse/Rat Peroxiredoxin 2 Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 06/10/2020
Human/Mouse/Rat Peroxiredoxin 2 Antibody

Name: Anonymous

Application: MiAr

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 11/09/2018
Human/Mouse/Rat Peroxiredoxin 2 Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 11/07/2018

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Human/Mouse/Rat Peroxiredoxin 2 Antibody

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Applications for Human/Mouse/Rat Peroxiredoxin 2 Antibody

Scientific Data Examples for Human/Mouse/Rat Peroxiredoxin 2 Antibody

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Peroxiredoxin 2

Citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody (1)

Customer Reviews for Human/Mouse/Rat Peroxiredoxin 2 Antibody (3)

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Human/Mouse/Rat Peroxiredoxin 2 Antibody

Human/Mouse/Rat Peroxiredoxin 2 Antibody

Human/Mouse/Rat Peroxiredoxin 2 Antibody

Product Documents for Human/Mouse/Rat Peroxiredoxin 2 Antibody

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