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Human/Mouse/Rat p38 alpha Antibody

Catalog # MAB869 | R&D Systems, Inc. a Bio-Techne Brand
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MAB869
MAB869-SP

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 142102

Product Summary for Human/Mouse/Rat p38 alpha Antibody

Immunogen

E. coli-derived recombinant human p38 alpha
Accession # Q16539

Specificity

Detects human, mouse, and rat p38 alpha. This antibody does not detect recombinant p38 beta, p38 gamma or p38 delta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat p38 alpha Antibody

Detection of Human/Mouse/Rat p38a antibody by Western Blot.

Detection of Human/Mouse/Rat p38 alpha by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat p38a Monoclonal Antibody (Catalog # MAB869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p38a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western Blot Shows Human p38a Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human p38 alpha Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38a knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat p38a Monoclonal Antibody (Catalog # MAB869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for p38a at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat p38 alpha Antibody

Application
Recommended Usage

Knockout Validated

p38 alpha is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in p38 alpha knockout HEK293T cell line.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB869 in the following applications:

Published Applications

Read 1 publication using MAB869 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 alpha

p38 alpha (also known as MAPK14 or SAPK2A) is a member of the p38 MAPK family which are activated by various environmental stresses and pro-inflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs) or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include transcription regulator ATF2, MEF2C, MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

References

  1. Han, J. et al. (1994) Science 265:808.
  2. Ge, B. et al. (2002) Science 295:1291.

Alternate Names

MAPK14, SAPK2a

Entrez Gene IDs

1432 (Human); 26416 (Mouse); 81649 (Rat)

Gene Symbol

MAPK14

UniProt

Product Documents for Human/Mouse/Rat p38 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat p38 alpha Antibody

For research use only

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