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Human/Mouse/Rat Olig2 Antibody Best Seller
Catalog # AF2418 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human/Mouse/Rat Olig2 Antibody
Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Met1-Lys323
Accession # Q13516
Specificity
Detects human Olig2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Olig2 Antibody
Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells.
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation.
A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.
Olig2 in Mouse Embryo.
Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat Olig2 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
Sample: A172 human glioblastoma cell line chromatin, p21 promoter detected by standard PCR.
Sample: A172 human glioblastoma cell line chromatin, p21 promoter detected by standard PCR.
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed 7 day differentiated rat cortical stem cells
Sample: Immersion fixed 7 day differentiated rat cortical stem cells
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13), human brain (cortex), and rat midbrain.
Sample: Immersion fixed frozen sections of mouse embryo (E13), human brain (cortex), and rat midbrain.
Western Blot
0.1 µg/mL
Sample: Recombinant Human Olig2
Sample: Recombinant Human Olig2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 6 reviews rated 4.8 using AF2418 in the following applications:
- Affinity Purification (1 Review)
- Immunocytochemistry (1 Review)
- Immunocytochemistry/Immunofluorescence (2 Reviews)
- Immunohistochemistry (1 Review)
- Immunohistochemistry-Frozen (1 Review)
Published Applications
Read 101 publications using AF2418 in the following applications:
- Bioassay (1 Publication)
- ChIP (1 Publication)
- Co-Immunoprecipitation (1 Publication)
- FISH (1 Publication)
- Flow Cytometry (2 Publications)
- ICC (21 Publications)
- ICC/IF (1 Publication)
- IHC (63 Publications)
- IHC-Fr (7 Publications)
- IHC-P (2 Publications)
- IHC/IF (1 Publication)
- Western Blot (9 Publications)
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Olig2
Product Documents for Human/Mouse/Rat Olig2 Antibody
Product Specific Notices for Human/Mouse/Rat Olig2 Antibody
For research use only
Citations for Human/Mouse/Rat Olig2 Antibody (101)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Olig2 Antibody include:
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Species: Mouse
Sample Types: Organoids
Applications: IHCAbdel Fattah et al. (2023-08-30), Targeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development Nature communications
PMID: 37644160 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCWalter et al. (2023-08-14), The Single-Dose Application of Interleukin-4 Ameliorates Secondary Brain Damage in the Early Phase after Moderate Experimental Traumatic Brain Injury in Mice International journal of molecular sciences
PMID: 37628939 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IFBaeza-Kallee et al. (2023-07-19), Deciphering the Action of Neuraminidase in Glioblastoma Models International journal of molecular sciences
PMID: 37511403 -
Species: Human
Sample Types: Nuclei,Tissue Homogenates
Applications: Flow Cytometry,Western BlotMätlik et al. (2023-06-08), Cell Type Specific CAG Repeat Expansions and Toxicity of Mutant Huntingtin in Human Striatum and Cerebellum bioRxiv : the preprint server for biology
PMID: 37333326 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCSeeker et al. (2023-05-22), Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and�CNS function Acta neuropathologica communications
PMID: 37217978 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCMaruyama et al. (2023-05-12), Free fatty acids support oligodendrocyte survival in a mouse model of amyotrophic lateral sclerosis Frontiers in cellular neuroscience
PMID: 37252191 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCZhang et al. (2023-05-05), TMEM106B regulates microglial proliferation and survival in response to demyelination Science advances
PMID: 37146150 -
Species: Human
Sample Types: Nuclei
Applications: ICCPressl et al. (2023-04-24), Layer 5a Corticostriatal Projection Neurons are Selectively Vulnerable in Huntington's Disease bioRxiv : the preprint server for biology
PMID: 37162977 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCY Wang et al. (2023-02-18), Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-kappaB pathway in LPC-induced demyelination Journal of Neuroinflammation, 2023-020(1):41.
PMID: 36803990 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryH Park et al. (2023-02-13), Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD mice Nature Communications, 2023-014(1):802.
PMID: 36781874 -
Species: Hamster - Mesocricetus auratus (Golden Hamster)
Sample Types: Whole Tissue
Applications: BioassayM Ferren et al. (2023-01-13), Early Permissiveness of Central Nervous System Cells to Measles Virus Infection Is Determined by Hyperfusogenicity and Interferon Pressure Viruses, 2023-015(1).
PMID: 36680268 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCS Chen et al. (2022-12-21), Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease Acta neuropathologica communications, 2022-110(1):188.
PMID: 36544231 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC/IFSI Ramos et al. (2022-12-12), An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics Nature Communications, 2022-113(1):7671.
PMID: 36509746 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotI Berglar et al. (2022-12-03), CHRDL1 Regulates Stemness in Glioma Stem-like Cells Cells, 2022-111(23).
PMID: 36497175 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCT Dierckx et al. (2022-11-07), Phloretin enhances remyelination by stimulating oligodendrocyte precursor cell differentiation Proceedings of the National Academy of Sciences of the United States of America, 2022-1119(46):e2120393119.
PMID: 36343243 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Su et al. (2022-11-03), A single-cell transcriptome atlas of glial diversity in the human hippocampus across the postnatal lifespan Cell Stem Cell, 2022-129(11):1594-1610.e8.
PMID: 36332572 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCT Zhou et al. (2022-10-24), Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice Nature Communications, 2022-113(1):6233.
PMID: 36280666 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCB Pai et al. (2022-10-23), High-resolution transcriptomics informs glial pathology in human temporal lobe epilepsy Acta neuropathologica communications, 2022-110(1):149.
PMID: 36274170 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCN Vilallongu et al. (2022-10-13), Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature Communications, 2022-113(1):6040.
PMID: 36229455 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Benraiss et al. (2022-08-30), A TCF7L2-responsive suppression of both homeostatic and compensatory remyelination in Huntington disease mice Cell Reports, 2022-040(9):111291.
PMID: 36044851 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCD Moezzi et al. (2022-07-26), Expression of antioxidant enzymes in lesions of multiple sclerosis and its models Scientific Reports, 2022-012(1):12761.
PMID: 35882921 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Zhou et al. (2022-07-06), Molecular landscapes of human hippocampal immature neurons across lifespan Nature, 2022-00(0).
PMID: 35794479 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCQ Liu et al. (2022-06-20), Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats Journal of Neuroinflammation, 2022-019(1):160.
PMID: 35725619 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCA Badia-Sote et al. (2022-04-22), High-Throughput Analysis of Astrocyte Cultures Shows Prevention of Reactive Astrogliosis by the Multi-Nutrient Combination Fortasyn Connect Cells, 2022-011(9).
PMID: 35563732 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: ICC,IHCC Azevedo et al. (2022-03-16), Parkinson's disease and multiple system atrophy patient iPSC-derived oligodendrocytes exhibit alpha-synuclein-induced changes in maturation and immune reactive properties Proceedings of the National Academy of Sciences of the United States of America, 2022-0119(12):e2111405119.
PMID: 35294277 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotT Feng et al. (2022-03-14), TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications, 2022-010(1):33.
PMID: 35287730 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCW Wang et al. (2022-02-17), The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development Nature Communications, 2022-013(1):958.
PMID: 35177643 -
Species: Human
Sample Types: Whole Cells
Applications: ICCT Xu et al. (2022-02-16), Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia EBioMedicine, 2022-077(0):103882.
PMID: 35182996 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCC Ogawa et al. (2022-02-16), BMP10 expression in the adult rat central nervous system Journal of chemical neuroanatomy, 2022-0121(0):102084.
PMID: 35182716 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCBD Needham et al. (2022-02-14), A gut-derived metabolite alters brain activity and anxiety behaviour in mice Nature, 2022-0602(7898):647-653.
PMID: 35165440 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCD Almansa et al. (2022-01-25), Extracellular Vesicles Derived from Young Neural Cultures Attenuate Astrocytic Reactivity In Vitro International Journal of Molecular Sciences, 2022-023(3).
PMID: 35163295 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCQ Wu et al. (2022-01-25), Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells Nature Communications, 2022-013(1):470.
PMID: 35078993 -
Species: Human
Sample Types: Whole Cells
Applications: ICCWU Park et al. (2021-12-01), A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) Biology, 2021-110(12).
PMID: 34943169 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCLM Toomey et al. (2021-11-19), Cuprizone feed formulation influences the extent of demyelinating disease pathology Scientific Reports, 2021-111(1):22594.
PMID: 34799634 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCY Kimura et al. (2021-10-26), IL-33 induces orofacial neuropathic pain through Fyn-dependent phosphorylation of GluN2B in the trigeminal spinal nucleus caudalis Brain, Behavior, and Immunity, 2021-10(0).
PMID: 34715301 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCY Takanezawa et al. (2021-09-08), Microglial ASD-related genes are involved in oligodendrocyte differentiation Scientific Reports, 2021-011(1):17825.
PMID: 34497307 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCLE Blizzard et al. (2021-07-07), A Novel Mutation in Cse1l Disrupts Brain and Eye Development with Specific Effects on Pax6 Expression Journal of developmental biology, 2021-09(3).
PMID: 34287339 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCM Connor et al. (2021-07-02), Nanoparticulate matter exposure results in white matter damage and an inflammatory microglial response in an experimental murine model PLoS ONE, 2021-016(7):e0253766.
PMID: 34214084 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCT Zhang et al. (2021-06-08), Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum Cell Reports, 2021-035(10):109208.
PMID: 34107249 -
Species: Human
Sample Types: Cell Lysates,Whole Tissue
Applications: Co-Immunoprecipitation,Western Blot,IHCP Filppu et al. (2021-05-10), CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity JCI Insight, 2021-06(9).
PMID: 33986188 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCW Mu et al. (2021-04-16), Hypothalamic Rax+ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice Nature Communications, 2021-012(1):2288.
PMID: 33863883 -
Species: Human
Sample Types: Cell Lysates,Whole Tissue
Applications: IHC,Western BlotJE Lee et al. (2021-04-08), Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells Scientific Reports, 2021-011(1):7778.
PMID: 33833342 -
Species: Human
Sample Types: Whole Cells
Applications: ICCBY Fan et al. (2021-03-01), Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4 Neural regeneration research, 2021-016(3):561-566.
PMID: 32985488 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCY Dong et al. (2021-02-18), Oxidized phosphatidylcholines found in multiple sclerosis lesions mediate neurodegeneration and are neutralized by microglia Nature Neuroscience, 2021-00(0).
PMID: 33603230 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCC Ogawa et al. (2021-02-11), BMP9 expression in the adult rat brain Journal of chemical neuroanatomy, 2021-0113(0):101933.
PMID: 33582251 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCL Poh et al. (2020-12-09), AIM2 inflammasome mediates hallmark neuropathological alterations and cognitive impairment in a mouse model of vascular dementia Molecular Psychiatry, 2020-10(0).
PMID: 33299135 -
Species: Mouse,Human
Sample Types: Whole Tissue
Applications: IHCW Tao et al. (2020-10-30), SATB2 drives glioblastoma growth by recruiting CBP to promote FOXM1 expression in glioma stem cells EMBO Mol Med, 2020-10(0):e12291.
PMID: 33124191 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotAL Koessinger et al. (2020-09-21), Quantitative in vivo bioluminescence imaging of orthotopic patient-derived glioblastoma xenografts Scientific Reports, 2020-010(1):15361.
PMID: 32958777 -
Species: Chicken,Mouse
Sample Types: Whole Tissue
Applications: IHCW Wang et al. (2020-09-15), PRC2 Acts as a Critical Timer That Drives Oligodendrocyte Fate over Astrocyte Identity by Repressing the Notch Pathway Cell Rep, 2020-032(11):108147.
PMID: 32937136 -
Species: Mouse,Transgenic Mouse
Sample Types: Whole Tissue
Applications: FISH,IHCMJ Borrett et al. (2020-08-11), Single-Cell Profiling Shows Murine Forebrain Neural Stem Cells Reacquire a Developmental State when Activated for Adult Neurogenesis Cell Rep, 2020-032(6):108022.
PMID: 32783944 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCS Kaji et al. (2020-07-29), BCAS1-positive immature oligodendrocytes are affected by the &alpha-synuclein-induced pathology of multiple system atrophy Acta Neuropathol Commun, 2020-08(1):120.
PMID: 32727582 -
Species: Mouse
Sample Types: Whole Cells,Whole Tissue
Applications: ICC,IHCM Duman et al. (2020-07-09), EEF1A1 deacetylation enables transcriptional activation of remyelination Nat Commun, 2020-011(1):3420.
PMID: 32647127 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCSM Nam et al. (2020-05-01), Ginseng gintonin alleviates neurological symptoms in the G93A-SOD1 transgenic mouse model of amyotrophic lateral sclerosis through lysophosphatidic acid 1 receptor Journal of Ginseng Research, 2020-045(3):390-400.
PMID: 34025132 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLM Ruiz-Perer et al. (2020-04-22), A Matter of Choice: Inhibition of c-Rel Shifts Neuronal to Oligodendroglial Fate in Human Stem Cells Cells, 2020-09(4).
PMID: 32331232 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCTC Ma et al. (2020-03-17), Spatiotemporal Decline of BMP Signaling Activity in Neural Progenitors Mediates Fate Transition and Safeguards Neurogenesis Cell Rep, 2020-030(11):3616-3624.e4.
PMID: 32187534 -
Species: Human
Sample Types: Whole Cells
Applications: ICCM Fares et al. (2020-03-03), Pathological modeling of TBEV infection reveals differential innate immune responses in human neurons and astrocytes that correlate with their susceptibility to infection J Neuroinflammation, 2020-017(1):76.
PMID: 32127025 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCSM Nam et al. (2020-03-02), Ginseng Gintonin Attenuates Lead-Induced Rat Cerebellar Impairments during Gestation and Lactation Biomolecules, 2020-010(3).
PMID: 32131481 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCC Ogawa et al. (2020-01-31), Follistatin expression in the central nervous system of the adult rat J. Chem. Neuroanat., 2020-0105(0):101753.
PMID: 32014555 -
Species: Mouse
Sample Types: Tissue
Applications: IHCY Zhou et al. (2020-01-13), Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease Nat. Med., 2020-026(1):131-142.
PMID: 31932797 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCS Clavreul et al. (2019-10-25), Cortical astrocytes develop in a plastic manner at both clonal and cellular levels Nat Commun, 2019-110(1):4884.
PMID: 31653848 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCLM Toomey et al. (2019-10-25), Comparing modes of delivery of a combination of ion channel inhibitors for limiting secondary degeneration following partial optic nerve transection Sci Rep, 2019-19(1):15297.
PMID: 31653948 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCM Boström et al. (2019-07-03), Chronic disturbance in the thalamus following cranial irradiation to the developing mouse brain Sci Rep, 2019-09(1):9588.
PMID: 31270437 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCG Gopalasing et al. (2019-06-08), The effects of a combination of ion channel inhibitors on pathology in a model of demyelinating disease Mult Scler Relat Disord, 2019-034(0):1-8.
PMID: 31202958 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-FrY Hayashi et al. (2019-03-27), BMP6 expression in the adult rat central nervous system J. Chem. Neuroanat., 2019-00(0).
PMID: 30928603 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCV Bellver-La et al. (2019-01-31), Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019-010(1):518.
PMID: 30705270 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCM Osipovitch et al. (2018-12-13), Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation Cell Stem Cell, 2018-10(0).
PMID: 30554964 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrAM Falcão et al. (2018-11-12), Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis Nat. Med., 2018-10(0).
PMID: 30420755 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLM Ruiz-Perer et al. (2018-10-30), NF-?B p65 directs sex-specific neuroprotection in human neurons Sci Rep, 2018-18(1):16012.
PMID: 30375448 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCY Hayashi et al. (2018-10-24), Myostatin expression in the adult rat central nervous system J. Chem. Neuroanat., 2018-10(0).
PMID: 30366029 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCQ Chen et al. (2018-09-18), Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate MBio, 2018-09(5).
PMID: 30228241 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrF Cathomas et al. (2018-04-17), Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice Genes Brain Behav., 2018-00(0):e12475.
PMID: 29566304 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCT Srivastava et al. (2018-04-16), A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors J. Clin. Invest., 2018-00(0).
PMID: 29664021 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrM Datta et al. (2018-03-13), Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner Immunity, 2018-00(0).
PMID: 29548672 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCW Zhou et al. (2017-11-02), Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy Cell Stem Cell, 2017-121(5):591-603.e4.
PMID: 29100012 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCFI Young et al. (2017-06-27), The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1 Proc. Natl. Acad. Sci. U.S.A., 2017-00(0).
PMID: 28655839 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCNJ Yates et al. (2017-02-01), Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors Neural Regen Res, 2017-012(2):307-316.
PMID: 28400815 -
Species: Human
Sample Types: Tissue Homogenates
Applications: IHC(2016-12-19), Brain tissue banking for stem cells for our future Sci Rep, 2016-16(0):39394.
PMID: 27991551 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCMajistor Raj Luxman Maglo Renkilaraj (2016-12-06), The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation Neurobiol. Dis, 2016-10(0).
PMID: 27940202 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICC(2016-08-23), Patched1 and Patched2 inhibit Smoothened non-cell autonomously Elife, 2016-05(0).
PMID: 27552050 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC(2016-08-18), A cord blood monocyte-derived cell therapy product accelerates brain remyelination JCI Insight, 2016-01(13):e86667.
PMID: 27699230 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotQi Xie (2016-06-20), RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation J Clin Invest, 2016-0126(7):2757-2772.
PMID: 27322055 -
Species: Human
Sample Types: Cell Lysates
Applications: ChIP(2016-05-06), Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression J Leukoc Biol, 2016-00(0).
PMID: 27154355 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PSH Choi et al. (2016-04-13), Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors PLoS ONE, 2016-011(4):e0153443.
PMID: 27074032 -
Species: Human
Sample Types: Cell Lysates
Mendes F et al. (2015-08-04), Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro. PLoS ONE, 2015-010(8):e0133689.
PMID: 26241738 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCRzem R et al. (2015-03-12), A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair. PLoS ONE, 2015-010(3):e0119540.
PMID: 25763823 -
Species: Human
Sample Types: Cell Lysates,Whole Tissue
Applications: Western Blot,IHCXie Q et al. (2015-03-02), Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci, 2015-018(4):501-10.
PMID: 25730670 -
Species: Human
Sample Types: Whole Cells
Applications: ICCBen-Shushan E et al. (2015-02-01), Notch signaling regulates motor neuron differentiation of human embryonic stem cells. Stem Cells, 2015-033(2):403-15.
PMID: 25335858 -
Species: Rat
Sample Types: Whole Cells
Applications: IHCBergsland M et al. (2014-09-01), Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells. Stem Cells, 2014-032(9):2539-49.
PMID: 24807147 -
Species: Human
Sample Types: Whole Cells,Cell Lysates,Whole Tissue
Applications: IHC,ICC,Western BlotLiu et al. (2014-05-16), Phage display discovery of novel molecular targets in glioblastoma-initiating cells. Cell Death Differ, 2014-021(8):1325-39.
PMID: 24832468 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCYan K et al. (2014-05-01), Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy. Genes Dev, 2014-028(10):1085-100.
PMID: 24788093 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCSeto Y et al. (2014-01-01), Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum. Nat Commun, 2014-05(0):3337.
PMID: 24535035 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-FrSrikanth et al. (2013-11-22), BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells. Mol Cancer Res, 2013-112(2):283-94.
PMID: 24269952 -
Species: Human
Sample Types: Whole Cells
Applications: ICCPiltti K et al. (2013-02-14), Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy. Stem Cells Transl Med, 2013-02(3):204-16.
PMID: 23413374 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCKuboyama et al. (2012-11-07), Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination. PLoS ONE, 2012-17(11):e48797.
PMID: 23144976 -
Species: Chicken
Sample Types: Whole Tissue
Applications: IHCZelinka C et al. (2012-09-04), The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1. PLoS ONE, 2012-07(9):e44477.
PMID: 22973454 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PMartinez-Cerdeno V et al. (2012-06-26), N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells. PLoS ONE, 2012-07(6):e39456.
PMID: 22745758 -
Species: Human
Sample Types: Whole Cells
Applications: ICCSalazar DL et al. (2010-08-18), Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model. PLoS ONE, 2010-05(8):e12272.
PMID: 20806064 -
Species: Human
Sample Types: Whole Cells
Applications: ICCWeber K et al. (2008-02-12), A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis. Mol. Ther., 2008-016(4):698-706.
PMID: 18362927 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrVue TY et al. (2007-11-01), Characterization of progenitor domains in the developing mouse thalamus. J. Comp. Neurol., 2007-1505(1):73-91.
PMID: 17729296 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCWidestrand A et al. (2007-07-12), Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice. Stem Cells, 2007-025(10):2619-27.
PMID: 17628017 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrBauer S et al. (2006-11-15), Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain. J. Neurosci., 2006-126(46):12089-99.
PMID: 17108182
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Customer Reviews for Human/Mouse/Rat Olig2 Antibody (6)
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Spinal cordSpecies: MouseVerified Customer | Posted 07/09/2019This antibody worked nicely 1:500 or 1:1000 of mouse spinal cord perfused with 4% PFA.
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Application: Immunohistochemistry-FrozenSample Tested: Mouse brainSpecies: MouseVerified Customer | Posted 05/12/2019
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Application: ImmunohistochemistrySample Tested: Adult brainSpecies: MouseVerified Customer | Posted 06/20/2018This antibody worked beautifully at 1:2000 in 30um free-floating sections of adult (P25) mouse brain perfused with 4% PFA and cryoprotected. Region imaged: corpus callosum above hippocampus. Protocol: 1. Antigen Retrieval: 100mM Sodium Citrate pH6.0, 0.05% Tween, in microwave 2. Rinse sections PBS once 3. Block in 5% NDS in PBSTx 1% RT for 1 hour 4. Primary Antibody (1:2000) in 1% NDS in PBSTx 0.3% (Incubate RT overnight on shaker) 5. Wash 3x 10 min PBS 6. Secondary Antibody (1:600) in 1% NDS in PBSTx 0.3% (Incubate RT 2h on shaker) 7. Wash 3x 10 min PBS 8. Mount on slides in water and let dry 9. Coverslip using Prolong Gold
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: GliomaSpecies: HumanVerified Customer | Posted 01/04/2018Glioma xenografted in NOD SCID mice -> olig2 in red
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Application: Affinity PurificationSample Tested: Brain sectionsSpecies: Rat Sprague DawleyVerified Customer | Posted 12/07/2015Dilution 1:500
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Application: ImmunocytochemistrySample Tested: See PMID 22504067Species: MouseVerified Customer | Posted 01/06/2015
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