Key Product Details
Product Summary for Human/Mouse/Rat Olig2 Antibody
Accession # Q13516
Scientific Data Images for Human/Mouse/Rat Olig2 Antibody
Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells.Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation.A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.
Olig2 in Mouse Embryo.Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Applications for Human/Mouse/Rat Olig2 Antibody
Chromatin Immunoprecipitation (ChIP)
Sample: A172 human glioblastoma cell line chromatin, p21 promoter detected by standard PCR.
Sample: Immersion fixed 7 day differentiated rat cortical stem cells
Sample: Immersion fixed frozen sections of mouse embryo (E13), human brain (cortex), and rat midbrain.
Sample: Recombinant Human Olig2
Read 6 reviews rated 4.8 using AF2418 in the following applications:
- Affinity Purification (1 Review)
- Immunocytochemistry (1 Review)
- Immunocytochemistry/Immunofluorescence (2 Reviews)
- Immunohistochemistry (1 Review)
- Immunohistochemistry-Frozen (1 Review)
Read 101 publications using AF2418 in the following applications:
- Bioassay (1 Publication)
- ChIP (1 Publication)
- Co-Immunoprecipitation (1 Publication)
- FISH (1 Publication)
- Flow Cytometry (2 Publications)
- ICC (21 Publications)
- ICC/IF (1 Publication)
- IHC (63 Publications)
- IHC-Fr (7 Publications)
- IHC-P (2 Publications)
- IHC/IF (1 Publication)
- Western Blot (9 Publications)
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Specific Notices for Human/Mouse/Rat Olig2 Antibody
For research use only