Human/Mouse/Rat JNK Pan Specific Antibody

JNK in Rat Kidney.

JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human JNK by Simple Western^TM.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human JNK1/2/3 by Western Blot

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human JNK1/2/3 by Western Blot

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human JNK1/2/3 by Western Blot

Detection of JNK activation in human fibroblasts and corals.Immunoblot revealing activated (pJNK) and total forms of JNK (JNK) present in (a) human fibroblasts and (b) S. pistillata nubbins incubated in the absence (control; ct) or presence of SP600125 (SP), anisomycin (Ani), or combination of both (Ani + SP). Molecular weight standards in kilodaltons (kDa) are indicated on the right side of the blots. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.