Human/Mouse/Rat iNOS Antibody

Detection of Mouse iNOS by Simple Western^TM.

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS, loaded at 0.2 mg/mL. A specific band was detected for iNOS at approximately 136 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Rat iNOS by Western Blot

Ultrasound (US) stimulation reduces ED-1, iNOS, and TNF-alpha expression in the acute spinal cord injury (SCI) phase. (A–C) ED-1 (macrophage/microglia, green) and iNOS (inducible nitric oxide synthase, red) were evaluated in the sham (A), SCIU40 (B), and SCIU5 (c) experimental groups at 7 days post-SCI (five sections per rat, n = 3). (D–F) Cross-sections were counterstained with anti-TNF-alpha (red) and ED-1 (green) antibodies (five sections per rat, n = 3). (G–I) Quantification of fluorescence intensity of ED-1, iNOS, and TNF-alpha. Two-way ANOVA with Turkey's test for multiple comparisons was used for analyses. *P < 0.05, ****P < 0.0001 compared with the sham control, #P < 0.05, ###P < 0.001, ####P < 0.0001 compared with the SCIU5-treated group. (J) Representative western blotting results for ED-1, iNOS, TNF-alpha, and GAPDH at 7 days post-SCI (Lane 1, sham; lane 2, SCIU40; lane 3, SCIU5;). Relative expression levels of ED-1, iNOS, and TNF-alpha were calculated after normalization to the level of GAPDH. Full-length blots are presented in Supplementary Fig. 8. Data are presented as means ± SEM. Two-tailed Student’s t-tests were used for comparison. *P < 0.05 compared with the sham control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35256617), licensed under a CC-BY license. Not internally tested by R&D Systems.