Human/Mouse/Rat iNOS Antibody
Catalog # MAB9502 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human/Mouse/Rat iNOS Antibody
Immunogen
Pro781-His798
Accession # P35228
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat iNOS Antibody
Detection of Human iNOS by Western Blot.
Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.Detection of Mouse and Rat iNOS by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.iNOS in Human Brain.
iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat iNOS Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla).
Simple Western
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS
Western Blot
Sample: DLD clone 2C2 human colon adenocarcinoma cell line treated with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # 201-LB, 210-TA, 285-IF), RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, and NR8383 rat alveolar macrophage cell line treated with LPS
Reviewed Applications
Read 8 reviews rated 4 using MAB9502 in the following applications:
Formulation, Preparation and Storage
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Reconstitution
Formulation
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Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: iNOS
Product Documents for Human/Mouse/Rat iNOS Antibody
Product Specific Notices for Human/Mouse/Rat iNOS Antibody
For research use only
Citations for Human/Mouse/Rat iNOS Antibody (30)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat iNOS Antibody include:
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Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotG Moço et al. (2023-01-23), Synthesis of Carvone Derivatives and In Silico and In Vitro Screening of Anti-Inflammatory Activity in Murine Macrophages International Journal of Molecular Sciences, 2023-024(3).
PMID: 36768590 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotB Saravi et al. (2022-10-28), In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells Cells, 2022-111(21).
PMID: 36359814 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCJY Hong et al. (2022-09-14), Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis Oxidative Medicine and Cellular Longevity, 2022-02022(0):3472443.
PMID: 36160714 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotT Kondo et al. (2022-04-16), Insulin-like growth factor 2 promotes osteoclastogenesis increasing inflammatory cytokine levels under hypoxia Journal of pharmacological sciences, 2022-0149(3):93-99.
PMID: 35641033 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotYR Hong et al. (2022-03-07), Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury Scientific Reports, 2022-012(1):3636.
PMID: 35256617 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotF Yu et al. (2022-01-16), Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages International immunopharmacology, 2022-0104(0):108514.
PMID: 35045357 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotH Kim et al. (2021-11-27), Gongjin-Dan Enhances Neurite Outgrowth of Cortical Neuron by Ameliorating H2O2-Induced Oxidative Damage via Sirtuin1 Signaling Pathway Nutrients, 2021-113(12).
PMID: 34959841 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCS Lim et al. (2021-11-04), Senolytic Therapy for Cerebral Ischemia-Reperfusion Injury International Journal of Molecular Sciences, 2021-122(21).
PMID: 34769397 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotY Wang et al. (2021-11-01), Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice Theranostics, 2021-111(20):10074-10090.
PMID: 34815805 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotL Liu et al. (2021-09-28), BCG immunotherapy inhibits cancer progression by promoting the M1 macrophage differentiation of THP?1 cells via the Rb/E2F1 pathway in cervical carcinoma Oncology reports, 2021-046(5).
PMID: 34581419 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCW Katagiri et al. (2021-08-31), Migration and phenotype switching of macrophages at early-phase of bone-formation by secretomes from bone marrow derived mesenchymal stem cells using rat calvaria bone defect model Journal of dental sciences, 2021-017(1):421-429.
PMID: 35028066 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCJY Hong et al. (2021-08-26), Neurotherapeutic Potential of Cervus elaphus Sibericus on Axon Regeneration and Growth Cone Reformation after H2O2-Induced Injury in Rat Primary Cortical Neurons Biology, 2021-010(9).
PMID: 34571710 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Chu et al. (2021-03-09), Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface Journal of reproductive immunology, 2021-0145(0):103309.
PMID: 33774530 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Vaamonde-G et al. (2021-01-26), Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis Carbohydrate polymers, 2021-0258(0):117692.
PMID: 33593565 -
Species: Mouse
Sample Types: Cell Lysates
A Silva et al. (2020-12-18), Anti-inflammatory potential of Portuguese thermal waters Scientific Reports, 2020-110(1):22313.
PMID: 33339881 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCLC Cheng et al. (2020-08-03), Cancer-Derived Transforming Growth Factor-beta Modulates Tumor-Associated Macrophages in Ampullary Cancer Onco Targets Ther, 2020-013(0):7503-7516.
PMID: 32821120 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Sousa et al. (2020-04-29), Standardised comparison of limonene-derived monoterpenes identifies structural determinants of anti-inflammatory activity Sci Rep, 2020-010(1):7199.
PMID: 32350292 -
Species: N/A
Sample Types: Recombinant Protein
Applications: Western BlotCP Faria et al. (2020-04-10), Giardia lamblia Decreases NF-&kappaB p65RelA Protein Levels and Modulates LPS-Induced Pro-Inflammatory Response in Macrophages Sci Rep, 2020-010(1):6234.
PMID: 32277133 -
Species: Mouse
Sample Types: Protein
Applications: Western BlotWC Liu et al. (2020-04-01), Supplementation of endogenous Ahr ligands reverses insulin resistance and associated inflammation in an insulin-dependent diabetic mouse model J. Nutr. Biochem., 2020-083(0):108384.
PMID: 32512500 -
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western BlotMAM Simões et al. (2020-02-13), Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes Molecules, 2020-025(4).
PMID: 32069907 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotC Ma et al. (2019-11-07), The pro-inflammatory effect of NR4A3 in osteoarthritis J. Cell. Mol. Med., 2019-10(0).
PMID: 31701670 -
Species: Xenograft
Sample Types: Cell Lysates
Applications: Western BlotW Ren et al. (2019-02-08), Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res., 2019-038(1):62.
PMID: 30736829 -
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHCS Altan et al. (2016-10-27), Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns Burns, 2016-143(1):232-244.
PMID: 28341026 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCYasuda Y et al. (2015-04-15), Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction. PLoS ONE, 2015-010(4):e0122458.
PMID: 25874769 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotXia N et al. (2014-03-24), Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells. Molecules, 2014-019(3):3654-68.
PMID: 24662080 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotDietrich S et al. (2013-10-18), High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy. Haematologica, 2013-199(3):541-7.
PMID: 24142995 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot(2012-05-23), Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012-013(0):50.
PMID: 22621398 -
Species: Human
Sample Types: Whole Cells,Whole Tissue
Applications: ICC,IHC-PEspinosa A et al. (2008-09-25), The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide. Exp. Cell Res., 2008-0314(20):3605-13.
PMID: 18845142 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PSkaleric U et al. (2006-10-02), Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease. Infect. Immun., 2006-174(12):7010-3.
PMID: 17015454 -
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-PYuan LJ et al. (2004-09-01), Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production. J. Orthop. Res., 2004-022(5):1126-34.
PMID: 15304289
There are no citations that match your criteria.
Customer Reviews for Human/Mouse/Rat iNOS Antibody (8)
Customer Images
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Application: ImmunohistochemistrySample Tested: Lung tissueSpecies: HumanVerified Customer | Posted 04/11/2023Leica Bond ER2 heat retrieval primary Ab MAB9502 at 5ug/mL in BSA-TBS Leica standard IHC protocol on Leica Rx stainer
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Application: ImmunohistochemistrySample Tested: Bone marrow tissueSpecies: MouseVerified Customer | Posted 08/13/2021
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Connective tissueSpecies: MouseVerified Customer | Posted 07/06/2021
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Application: Western BlotSample Tested: RAW 264.7 mouse monocyte/macrophage cell lineSpecies: Mouse and HumanVerified Customer | Posted 04/03/2021Antibody also works very well for immunohistochemistry
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Application: ImmunohistochemistrySample Tested: Adult brainSpecies: RatVerified Customer | Posted 07/28/2020Rats were perfused with 4% paraformaldehyde in PBS (pH=7.6). The brain was removed and immersed in PFA then in 30% sucrose. The brain was sliced with a freezing microtome. A mixture of 1% Triton-X and 10% donkey serum (in TRIS) was used for antigen retrieval and blocking. Tmem119 antibody was used in 1:100 dilution and was visualized by a Cy3 conjugated anti-mouse secondary antibody (1:500, yellow). Cell bodies were stained with NeuroTrace 640/660 (1:250, red). Pictures show a part of the rat hippocampus. The product was assumed to label activated microglial cells. It seems, it did not. However, it labelled some cerebral blood vessels, which is also meaningful, thus, it may be useful for this application.
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Application: Simple WesternSample Tested: skin wound - diabeticSpecies: RatVerified Customer | Posted 02/12/2020Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
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Application: Western BlotSample Tested: See PMID 21075192Species: MouseVerified Customer | Posted 02/25/2015
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Application: Immunohistochemistry-ParaffinSample Tested: See PMID 23147558Species: HumanVerified Customer | Posted 02/25/2015
There are no reviews that match your criteria.
FAQs for Human/Mouse/Rat iNOS Antibody
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Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
A: We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
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