Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human APE by Simple Western^TM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for APE at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of APE in A549 human lung carcinoma cells.

APE was detected in immersion fixed A549 human lung carcinoma cells using Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human/Mouse/Rat APE Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cells (Positive) and HepG2 human hepatocellular carcinoma cells (Positive)

Simple Western

10 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APE

Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF-kappa B, and p53.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Entrez Gene IDs

328 (Human); 305605 (Rat)

Gene Symbol

APEX1

UniProt

P27695

Additional APE Products

Product Documents for Human/Mouse/Rat APE Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat APE Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat APE Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1044

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all APE Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat APE Antibody

Detection of Human, Mouse, and Rat APE by Western Blot.

Detection of Human APE by Simple WesternTM.