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Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody Best Seller

Catalog # MAB1420 | R&D Systems, Inc. a Bio-Techne Brand
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MAB1420
MAB1420-SP

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1A4

Product Summary for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody

Immunogen

Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha-smooth muscle actin (1)

Specificity

Detects human  alpha-Smooth Muscle Actin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody

Detection of Human a-Smooth Muscle Actin antibody by Western Blot.

Detection of Human alpha‑Smooth Muscle Actin by Western Blot.

Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat a-Smooth Muscle Actin antibody by Western Blot.

Detection of Mouse and Rat alpha‑Smooth Muscle Actin by Western Blot.

Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a-Smooth Muscle Actin antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

alpha‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells.

a-Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/ Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Dual RNAscope ISH-IHC Compatible

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart and human breast cancer tissue

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Simple Western

10 µg/mL
Sample: iBJ6 human induced pluripotent stem cell line differentiated to cardiomyocytes

Western Blot

0.1-0.25 µg/mL
Sample: Human heart tissue, cardiac differentiated iBJ6 human induced pluripotent stem cell line, mouse colon tissue, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and rat colon tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 13 reviews rated 4.9 using MAB1420 in the following applications:

Published Applications

Read 87 publications using MAB1420 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Smooth Muscle Actin

alpha‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References

  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Entrez Gene IDs

59 (Human); 11475 (Mouse); 81633 (Rat)

Gene Symbol

ACTA2

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody

For research use only

Citations for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody (87)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody include:

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Customer Reviews (13)

4.9 out of 5
13 customer ratings
5 stars
92%
4 stars
8%
3 stars
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  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 08/24/2023
    Works well at a dilution of 1:500 for IF experiments
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Endothelial cells
    Species: Mouse
    Verified Customer | Posted 10/19/2021
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Testicular tissue
    Species: Mouse
    Verified Customer | Posted 08/10/2021
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Muscle tissue
    Species: Mouse
    Verified Customer | Posted 08/04/2021
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/19/2019
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/21/2018
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 12/28/2017
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Li Liu
    Application: Western Blot
    Sample Tested: Skin tissue
    Species: Mouse
    Verified Customer | Posted 10/20/2017
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
    Name: Xiaoming Zhou
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 10/20/2017
    The antibody works well for lung tissue of mouse.
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Human alpha -Smooth Muscle Actin Antibody
    Name: Sandra Lourenssen
    Sample Tested: IHC on paraffin sections
    Species: Rat
    Verified Customer | Posted 03/03/2017
    Human/Mouse/Rat  alpha‑Smooth Muscle Actin Antibody MAB1420
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 20682625
    Species: Human
    Verified Customer | Posted 02/10/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 20682625
    Species: Human
    Verified Customer | Posted 02/10/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 20682625
    Species: Other
    Verified Customer | Posted 02/10/2015

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FAQs for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody

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  • Q: Human/Mouse/Rat alpha-Smooth Muscle Actin Antibody, Catalog # MAB1420, is validated for Human/Mouse/Rat IHC, but the datasheet only has Human IHC examples. Was there testing on Mouse and Rat IHC samples?

    A: Mouse and Rat samples were tested in Western Blot. We did not perform testing with Mouse or Rat IHC samples, however there are several citations for IHC using Mouse and Rat tissue on our webpage. 

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