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Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody Best Seller
Catalog # MAB1420 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1A4
Product Summary for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody
Immunogen
Peptide corresponding to
residues EEEDSTALVC of the N-term of human alpha-smooth muscle actin (1)
Specificity
Detects human alpha-Smooth Muscle Actin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody
Detection of Human alpha‑Smooth Muscle Actin by Western Blot.
Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat alpha‑Smooth Muscle Actin by Western Blot.
Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells.
a-Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/ Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Dual RNAscope ISH-IHC Compatible
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart and human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human heart and human breast cancer tissue
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Simple Western
10 µg/mL
Sample: iBJ6 human induced pluripotent stem cell line differentiated to cardiomyocytes
Sample: iBJ6 human induced pluripotent stem cell line differentiated to cardiomyocytes
Western Blot
0.1-0.25 µg/mL
Sample: Human heart tissue, cardiac differentiated iBJ6 human induced pluripotent stem cell line, mouse colon tissue, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and rat colon tissue
Sample: Human heart tissue, cardiac differentiated iBJ6 human induced pluripotent stem cell line, mouse colon tissue, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and rat colon tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 13 reviews rated 4.9 using MAB1420 in the following applications:
- Flow Cytometry (2 Reviews)
- Immunocytochemistry/Immunofluorescence (2 Reviews)
- Immunofluorescence (1 Review)
- Immunohistochemistry (3 Reviews)
- Western Blot (4 Reviews)
Published Applications
Read 87 publications using MAB1420 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Smooth Muscle Actin
alpha‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
References
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Alternate Names
AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA
Gene Symbol
ACTA2
Product Specific Notices for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody
For research use only
Citations for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody (87)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IHCShen et al. (2023-08-18), Iguratimod attenuated fibrosis in systemic sclerosis via targeting early growth response 1 expression Arthritis research & therapy
PMID: 37596660 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotRM Samsonraj et al. (2023-03-21), An unbiased proteomics approach to identify the senescence-associated secretory phenotype of human bone marrow-derived mesenchymal stem cells Bone Reports, 2023-018(0):101674.
PMID: 36994454 -
Species: Human
Sample Types: Protein,Whole Cells
Applications: ICC,Western BlotM Liu et al. (2023-01-20), Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis PLoS ONE, 2023-018(1):e0280385.
PMID: 36662769 -
Species: Human
Sample Types: Whole Cells
Applications: ICCD Karakas et al. (2023-01-19), Targeting Periostin Expression Makes Pancreatic Cancer Spheroids More Vulnerable to Natural Killer Cells Biomedicines, 2023-011(2).
PMID: 36830807 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot,IHCFM Suk et al. (2023-01-11), Dietary oxidized frying oil activates hepatic stellate cells and accelerates the severity of carbon tetrachloride- and thioacetamide-induced liver fibrosis in mice The Journal of nutritional biochemistry, 2023-00(0):109267.
PMID: 36641072 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotW Zeng et al. (2022-11-22), CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-10(0).
PMID: 36418470 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotD Zheng et al. (2022-11-03), Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment Bioactive materials, 2022-122(0):567-587.
PMID: 36382024 -
Species: Human
Sample Types: Whole Cells
Applications: ICCM New-Aaron et al. (2022-07-14), Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation Biology, 2022-011(7).
PMID: 36101437 -
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow CytometryJ Lotila et al. (2022-07-11), Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient Oncogene, 2022-063(0):102865.
PMID: 35843021 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCJH Wang et al. (2022-06-09), AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models Oncogene, 2022-012(1):9525.
PMID: 35680963 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCH Maruoka et al. (2022-05-15), Histochemical examination of blood vessels in murine femora with intermittent PTH administration Journal of oral biosciences, 2022-00(0).
PMID: 35584768 -
Species: Human
Sample Types: Spheroid
Applications: ICCY Shimoda et al. (2022-04-27), Generation of a human induced pluripotent stem cell line derived from a patient with dilated cardiomyopathy carrying LMNA nonsense mutation Stem Cell Research, 2022-062(0):102793.
PMID: 35500377 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotT Sakai et al. (2022-04-27), Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells International Journal of Molecular Sciences, 2022-023(9).
PMID: 35563212 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCV Crenn et al. (2022-04-15), Signature of the vascular tumor microenvironment as a marker of the therapeutic response to doxorubicin in a preclinical model of osteosarcoma American journal of cancer research, 2022-012(4):1843-1854.
PMID: 35530297 -
Species: Human
Sample Types: Whole Cells,Whole Tissue
Applications: IHC,CyTofTB Layton et al. (2022-03-23), A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease Proceedings of the National Academy of Sciences of the United States of America, 2022-0119(13):e2120336119.
PMID: 35320046 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMAS de Toledo et al. (2022-03-01), CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout Stem Cell Research, 2022-060(0):102732.
PMID: 35279545 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Western BlotRH Pires et al. (2022-02-01), Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation Physiological Reports, 2022-010(3):e15171.
PMID: 35166060 -
Species: Human
Sample Types: Whole Cells
Applications: ICCSF Mause et al. (2022-01-14), Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022-023(2).
PMID: 35055054 -
Species: Mouse
Sample Types: Spheroids
Applications: ICCT Kazi et al. (2022-01-07), Dermal Papilla Cell-Derived Extracellular Vesicles Increase Hair Inductive Gene Expression in Adipose Stem Cells via beta-Catenin Activation Cells, 2022-011(2).
PMID: 35053317 -
Species: Rat,Mouse
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,ICCK Hira et al. (2021-12-29), Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction) International immunopharmacology, 2021-1103(0):108494.
PMID: 34973530 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCY Luo et al. (2021-10-22), Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development Stem Cell Research, 2021-157(0):102583.
PMID: 34710837 -
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHCZ Li et al. (2021-07-29), Synergistic effects of silver nanoparticles and cisplatin in combating inflammation and hyperplasia of airway stents Bioactive materials, 2021-09(0):266-280.
PMID: 34820570 -
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow CytometryJWS Jahng et al. (2021-03-12), Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene Stem Cell Research, 2021-053(0):102284.
PMID: 33743362 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization,ICCY Wang et al. (2021-02-26), Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/&beta-catenin/PPAR&delta signalling Cell Death & Disease, 2021-012(2):214.
PMID: 33637678 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCC Jaworek et al. (2021-02-10), Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma Journal of personalized medicine, 2021-011(2).
PMID: 33578644 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Geuder et al. (2021-02-10), A non-invasive method to generate induced pluripotent stem cells from primate urine Scientific Reports, 2021-011(1):3516.
PMID: 33568724 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Western BlotMR Vitale et al. (2021-01-11), Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9 Stem Cell Research, 2021-051(0):102169.
PMID: 33486346 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCL Jin et al. (2020-11-23), STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells Nat Commun, 2020-111(1):5941.
PMID: 33230114 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCBB Patai et al. (2020-09-21), Initiation of reticular and spider veins, incompetent perforantes and varicose veins in the saphenous vein network of the rat Scientific Reports, 2020-010(1):15381.
PMID: 32958828 -
Species: Human
Sample Types: Whole Tissues
Applications: IHCS Ashwinbala et al. (2020-06-23), SB772077B (SB77) Alleviated the Aqueous Outflow Resistance Mediated by Cyclic Mechanical Stress in Perfused Human Cadaveric Eyes Sci Rep, 2020-010(1):10202.
PMID: 32576873 -
Species: Rat
Sample Types: Whole Tissues
Applications: IHCTT Ross et al. (2020-06-09), Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems Cell Mol Gastroenterol Hepatol, 2020-00(0).
PMID: 32526482 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Western BlotJW Blanchard et al. (2020-06-08), Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes Nat. Med., 2020-026(6):952-963.
PMID: 32514169 -
Species: Human
Sample Types: Whole Cells
Applications: CyTofTB Layton et al. (2020-06-02), Cellular census of human fibrosis defines functionally distinct stromal cell types and states Nat Commun, 2020-011(1):2768.
PMID: 32488016 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCC Liang et al. (2020-03-04), CD8+ T-cell plasticity regulates vascular regeneration in type-2 diabetes Theranostics, 2020-010(9):4217-4232.
PMID: 32226549 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCX Li et al. (2020-01-25), Dynamic Changes in Function and Proteomic Composition of Extracellular Vesicles from Hepatic Stellate Cells during Cellular Activation Cells, 2020-09(2).
PMID: 31991791 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCT Kazi et al. (2019-12-28), Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers FEBS Open Bio, 2019-10(0).
PMID: 31730301 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotNA Das et al. (2019-12-17), Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-to-mesenchymal transition Cell. Signal., 2019-168(0):109506.
PMID: 31862399 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: ICC,IHC-FrTW Kragstrup et al. (2019-12-02), Fibroblast-like synovial cell production of extra domain A fibronectin associates with inflammation in osteoarthritis BMC Rheumatol, 2019-13(0):46.
PMID: 31819923 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotRS Lindoso et al. (2019-11-15), Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by Promoting Cardio-Renal Protection Mol Ther Methods Clin Dev, 2019-116(0):63-77.
PMID: 31871958 -
Species: Rat
Sample Types: Whole Tissue,Tissue Homogenates
Applications: Western Blot,IHC-PM Komai et al. (2019-08-30), Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat Vet Sci, 2019-06(3).
PMID: 31480326 -
Species: Human
Sample Types: Whole Cells
Applications: ICCT Bikkuzin et al. (2019-02-15), Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells Stem Cell Res, 2019-037(0):101409.
PMID: 31121475 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PCF Guerrero-J et al. (2019-02-08), Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds Nat Commun, 2019-010(1):650.
PMID: 30737373 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCT Masuda et al. (2019-02-07), Inhibition of PAI-1 limits chemotherapy resistance in lung cancer through suppressing myofibroblast characteristics of cancer-associated fibroblasts J. Cell. Mol. Med., 2019-00(0).
PMID: 30734495 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western BlotA Kanda et al. (2019-01-24), TGF-?-SNAIL axis induces M�ller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane Sci Rep, 2019-09(1):673.
PMID: 30679596 -
S Kaur et al. (2018-10-19), Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 2018-18(1):15503.
PMID: 30341351 -
Sample Types: Whole Tissue
Applications: IHC-PRB Reisler et al. (2018-09-20), Ebola Virus Causes Intestinal Tract Architectural Disruption and Bacterial Invasion in Non-Human Primates Viruses, 2018-010(10).
PMID: 30241284 -
Species: Human
Sample Types: Tissue Homogenates,Whole Tissue
Applications: Western Blot,IHCQ Li et al. (2018-08-28), Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition Cell Death Dis, 2018-09(9):854.
PMID: 30154401 -
Species: Human
Sample Types: Organoids
Applications: IHCJ Barnes et al. (2018-08-15), Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells Mol Autism, 2018-09(0):44.
PMID: 30147856 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCO Espitia et al. (2018-01-26), Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification PLoS ONE, 2018-013(1):e0191976.
PMID: 29373585 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMH Kwack et al. (2018-01-10), Establishment and characterization of five immortalized human scalp dermal papilla cell lines Biochem. Biophys. Res. Commun., 2018-00(0).
PMID: 29331373 -
Species: Human
Sample Types: Whole Cells
Applications: ICCDE Reichman et al. (2018-01-08), Wnt inhibition promotes vascular specification of embryonic cardiac progenitors Development, 2018-0145(1).
PMID: 29217753 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotL Zhou et al. (2017-12-19), Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels Mol Med Rep, 2017-10(0).
PMID: 29257347 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCM Lombardi et al. (2017-07-07), P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels Sci Rep, 2017-07(1):4872.
PMID: 28687781 -
Species: Human
Sample Types: Cell Lysates,Whole Tissue
Applications: Western Blot,IHCH Kamata et al. (2017-07-04), KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis Sci Rep, 2017-07(1):4556.
PMID: 28676645 -
Species: Human
Sample Types: Whole Cells
Applications: ICCRCB Wong et al. (2017-05-19), Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors Stem Cell Res, 2017-022(0):13-15.
PMID: 28952926 -
Species: Human
Sample Types: Whole Cells
Applications: ICCRCB Wong et al. (2017-04-01), Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy Aging (Albany NY), 2017-09(4):1341-1350.
PMID: 28455970 -
Species: Canine
Sample Types: Whole Tissue
Applications: IHCSofia Avnet (2017-03-15), Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-?B acivation Int. J. Cancer, 2017-00(0).
PMID: 27888521 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PY Dong et al. (2017-03-09), Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane Sci Rep, 2017-07(0):44266.
PMID: 28276504 -
Species: Human
Sample Types: Whole Cells
Applications: ICCSC Hrstka et al. (2017-03-05), NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis Stem Cells, 2017-00(0).
PMID: 28142228 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCH Takahashi et al. (2017-01-31), Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages Oncotarget, 2017-08(5):8633-8647.
PMID: 28052009 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PDR Smith et al. (2017-01-09), Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon PLoS Negl Trop Dis, 2017-011(1):e0005296.
PMID: 28068342 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC(2016-11-18), Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment Cancer Res., 2016-10(0).
PMID: 27864347 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P(2016-11-03), The influence of warm ischemia elimination on kidney injury during transplantation - clinical and molecular study Sci Rep, 2016-16(0):36118.
PMID: 27808277 -
Species: Mouse
Sample Types: Whole Cells
Applications: IHCJian-Yong Xu (2016-07-07), Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration Stem Cells, 2016-00(0).
PMID: 27333945 -
Species: Human
Sample Types: Whole Cells
Applications: IHCW Qin et al. (2016-04-08), Exosomes in Human Breast Milk Promote EMT Clin Cancer Res, 2016-00(0).
PMID: 27060153 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PRiordan D et al. (2015-07-15), Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015-010(7):e0128975.
PMID: 26176839 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PMartinez L et al. (2015-03-24), CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients. PLoS ONE, 2015-010(3):e0121421.
PMID: 25803686 -
Species: Human
Sample Types: Whole Cells
Applications: ICCPan X et al. (2015-02-08), Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes. Int J Med Sci, 2015-012(3):248-55.
PMID: 25678842 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCDavaine J et al. (2014-09-26), Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. PLoS ONE, 2014-09(9):e107642.
PMID: 25259713 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PLiu T et al. (2014-06-11), Association between PON1 rs662 polymorphism and coronary artery disease. Eur J Clin Nutr, 2014-068(9):1029-35.
PMID: 24918121 -
Species: Human
Sample Types: Whole Cells
Applications: ICCPiao Y et al. (2014-06-05), Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors. Stem Cells Transl Med, 2014-03(0):787-791.
PMID: 24904173 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PDi Caro G et al. (2014-02-12), Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers. Clin Cancer Res, 2014-020(8):2147-58.
PMID: 24523438 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blotvan der Veer E et al. (2013-08-20), Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype. Circ Res, 2013-0113(9):1065-75.
PMID: 23963726 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMilani C et al. (2013-03-15), Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture. BMC Cancer, 2013-013(0):119.
PMID: 23497279 -
Species: Human
Sample Types: Whole Cells
Applications: ICCSong S et al. (2013-02-28), Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells. Exp Cell Res, 2013-0319(8):1136-45.
PMID: 23458169 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMohamed SA et al. (2012-06-18), Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve. Cardiol Res Pract, 2012-02012(0):165957.
PMID: 22745920 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMohamed SA et al. (2010-04-11), Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve. Ann. Anat., 2010-0192(3):145-50.
PMID: 20427168 -
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-POhshima S et al. (2010-03-23), Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging. J. Am. Coll. Cardiol., 2010-055(12):1240-9.
PMID: 20298932 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCZhang F et al. (2010-03-03), Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts. Am. J. Physiol., Cell Physiol., 2010-0298(5):C1209-16.
PMID: 20200208 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKim MH et al. (2009-11-14), The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices. Biomaterials, 2009-131(6):1213-8.
PMID: 19914711 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCZhou G et al. (2009-10-02), Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-1297(6):L1120-30.
PMID: 19801454 -
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHCFujimoto S et al. (2008-12-02), Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy. J. Am. Coll. Cardiol., 2008-152(23):1847-57.
PMID: 19038682 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotChetty A et al. (2008-07-25), Role of matrix metalloprotease-9 in hyperoxic injury in developing lung. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-0295(4):L584-92.
PMID: 18658276 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCHawsawi NM et al. (2008-04-15), Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes. Cancer Res., 2008-068(8):2717-25.
PMID: 18413739 -
Species: Human
Sample Types: Whole Cells
Applications: ICCEdward M et al. (2008-04-01), Gadodiamide contrast agent 'activates' fibroblasts: a possible cause of nephrogenic systemic fibrosis. J. Pathol., 2008-0214(5):584-93.
PMID: 18220317 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: Western Blot,ICCHong KM et al. (2007-06-07), Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma. J. Biol. Chem., 2007-0282(31):22910-20.
PMID: 17556364 -
Species: Human
Sample Types: Whole Cells
Applications: ICCSynenki L et al. (2007-03-01), Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation. Crit. Care Med., 2007-035(3):842-8.
PMID: 17255860
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Customer Reviews (13)
4.9 out of 5
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Mesenchymal stem cellsSpecies: HumanVerified Customer | Posted 08/24/2023Works well at a dilution of 1:500 for IF experiments
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Application: Western BlotSample Tested: Endothelial cellsSpecies: MouseVerified Customer | Posted 10/19/2021
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Application: ImmunohistochemistrySample Tested: Testicular tissueSpecies: MouseVerified Customer | Posted 08/10/2021
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Application: ImmunohistochemistrySample Tested: Muscle tissueSpecies: MouseVerified Customer | Posted 08/04/2021
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Application: ImmunohistochemistrySample Tested: Breast cancer tissueSpecies: HumanVerified Customer | Posted 02/19/2019
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Application: Western BlotSample Tested: HEK293 human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 07/21/2018
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: HMVEC human microvascular endothelial cellsSpecies: HumanVerified Customer | Posted 12/28/2017
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Application: Western BlotSample Tested: Skin tissueSpecies: MouseVerified Customer | Posted 10/20/2017
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Application: Western BlotSample Tested: Lung tissueSpecies: MouseVerified Customer | Posted 10/20/2017The antibody works well for lung tissue of mouse.
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Sample Tested: IHC on paraffin sectionsSpecies: RatVerified Customer | Posted 03/03/2017
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Application: ImmunofluorescenceSample Tested: See PMID 20682625Species: HumanVerified Customer | Posted 02/10/2015
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Application: Flow CytometrySample Tested: See PMID 20682625Species: HumanVerified Customer | Posted 02/10/2015
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Application: Flow CytometrySample Tested: See PMID 20682625Species: OtherVerified Customer | Posted 02/10/2015
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FAQs for Human/Mouse/Rat alpha‑Smooth Muscle Actin Antibody
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Q: Human/Mouse/Rat alpha-Smooth Muscle Actin Antibody, Catalog # MAB1420, is validated for Human/Mouse/Rat IHC, but the datasheet only has Human IHC examples. Was there testing on Mouse and Rat IHC samples?
A: Mouse and Rat samples were tested in Western Blot. We did not perform testing with Mouse or Rat IHC samples, however there are several citations for IHC using Mouse and Rat tissue on our webpage.
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