Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
Brand: rnd
SKU: MAB1420
Rating: 5 (8 reviews)

Catalog # MAB1420

Citations (60)

(8)

Datasheet / COA / SDS

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
Applications:	CyTOF-ready, Dual RNAscope ISH-IHC, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 1A4
Specificity:	Detects human alpha -Smooth Muscle Actin.

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Applications

	Recommended Concentration	Sample
Western Blot	0.1-0.25 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Dual RNAscope ISH-IHC	0.5-25 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody Scientific Data Examples

	Western Blot Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Western Blot Detection of Mouse and Rat alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. a-Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/ Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry alpha ‑Smooth Muscle Actin in Human Heart. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Intracellular Staining by Flow Cytometry Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
	Simple Western Detection of Human alpha ‑Smooth Muscle Actin by Simple Western^TM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Dual RNAscope ISH-IHC Compatible alpha-Smooth Muscle Actin in Human Breast Cancer Tissue Using Dual RNAscope^®ISH and IHC. alpha-Smooth Muscle Actin mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 444771-C2) and stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse/Rat alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog# MAB1420) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

Item	Value
References	Skalli, O. et al. (1986) J. Cell Biol. 103:2787. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name	Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs	59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names	AAT6, ACTA2, ACTSA, ACTVS, Actin alpha 2, Cell growth-inhibiting gene 46 protein, MYMY5, SMA, a-sma, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-SMA, alpha-Smooth Muscle Actin, alpha-actin-2, alphaSmooth Muscle Actin, growth-inhibiting gene 46, smooth muscle actin

Citations (60)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Wang et al. (2021), Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/&beta-catenin/PPAR&delta signalling Cell Death Disease, 2021;12(2):214.
PMID: 33637678
Species: Human
Sample Types: Whole Tissue
Applications: IHC

C Jaworek et al. (2021), Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma Journal of personalized medicine, 11(2):.
PMID: 33578644
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Geuder et al. (2021), A non-invasive method to generate induced pluripotent stem cells from primate urine Scientific Reports, 11(1):3516.
PMID: 33568724
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

JWS Jahng et al. (2021), Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene Stem Cell Research, 53(0):102284.
PMID: 33743362
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

BB Patai et al. (2020), Initiation of reticular and spider veins, incompetent perforantes and varicose veins in the saphenous vein network of the rat Scientific Reports, 10(1):15381.
PMID: 32958828
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

L Jin et al. (2020), STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells Nat Commun, 11(1):5941.
PMID: 33230114
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

C Liang et al. (2020), CD8+ T-cell plasticity regulates vascular regeneration in type-2 diabetes Theranostics, 10(9):4217-4232.
PMID: 32226549
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

X Li et al. (2020), Dynamic Changes in Function and Proteomic Composition of Extracellular Vesicles from Hepatic Stellate Cells during Cellular Activation Cells, 9(2):.
PMID: 31991791
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

RS Lindoso et al. (2020), Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by Promoting Cardio-Renal Protection Mol Ther Methods Clin Dev, 16(0):63-77.
PMID: 31871958
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Bikkuzin et al. (2019), Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells Stem Cell Res, 37(0):101409.
PMID: 31121475
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Kanda et al. (2019), TGF-?-SNAIL axis induces Mï¿½ller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane Sci Rep, 9(1):673.
PMID: 30679596
Species: Human
Sample Types: Whole Tissue
Applications: IHC

T Masuda et al. (2019), Inhibition of PAI-1 limits chemotherapy resistance in lung cancer through suppressing myofibroblast characteristics of cancer-associated fibroblasts J. Cell. Mol. Med., 0(0):.
PMID: 30734495
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

CF Guerrero-J et al. (2019), Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds Nat Commun, 10(1):650.
PMID: 30737373
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

M Komai et al. (2019), Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat Vet Sci, 6(3):.
PMID: 31480326
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

NA Das et al. (2019), Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-to-mesenchymal transition Cell. Signal., 68(0):109506.
PMID: 31862399
Species: Human
Sample Types: Whole Cells
Applications: ICC

TW Kragstrup et al. (2019), Fibroblast-like synovial cell production of extra domain A fibronectin associates with inflammation in osteoarthritis BMC Rheumatol, 3(0):46.
PMID: 31819923
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

T Kazi et al. (2019), Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers FEBS Open Bio, 0(0):.
PMID: 31730301
Species: Human
Sample Types: Whole Cells
Applications: ICC

DE Reichman et al. (2018), Wnt inhibition promotes vascular specification of embryonic cardiac progenitors Development, 145(1):.
PMID: 29217753
Species: Human
Sample Types: Whole Cells
Applications: ICC

MH Kwack et al. (2018), Establishment and characterization of five immortalized human scalp dermal papilla cell lines Biochem. Biophys. Res. Commun., 0(0):.
PMID: 29331373
Species: Primate - Macaca mulatta (Rhesus Macaques)
Sample Types: Whole Tissue
Applications: IHC-P

RB Reisler et al. (2018), Ebola Virus Causes Intestinal Tract Architectural Disruption and Bacterial Invasion in Non-Human Primates Viruses, 10(10):.
PMID: 30241284
Species: Human
Sample Types: Whole Tissue
Applications: IHC

O Espitia et al. (2018), Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification PLoS ONE, 13(1):e0191976.
PMID: 29373585
S Kaur et al. (2018), Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 8(1):15503.
PMID: 30341351
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Q Li et al. (2018), Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition Cell Death Dis, 9(9):854.
PMID: 30154401
Species: Human
Sample Types: Organoids
Applications: IHC

J Barnes et al. (2018), Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells Mol Autism, 9(0):44.
PMID: 30147856
Species: Human
Sample Types: Whole Cells
Applications: ICC

RCB Wong et al. (2017), Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy Aging (Albany NY), 9(4):1341-1350.
PMID: 28455970
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

DR Smith et al. (2017), Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon PLoS Negl Trop Dis, 11(1):e0005296.
PMID: 28068342
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Y Dong et al. (2017), Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane Sci Rep, 7(0):44266.
PMID: 28276504
Species: Human
Sample Types: Whole Cells
Applications: ICC

SC Hrstka et al. (2017), NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis Stem Cells, 0(0):.
PMID: 28142228
Species: Human
Sample Types: Whole Tissue
Applications: IHC

H Takahashi et al. (2017), Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages Oncotarget, 8(5):8633-8647.
PMID: 28052009
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

L Zhou et al. (2017), Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels Mol Med Rep, 0(0):.
PMID: 29257347
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Lombardi et al. (2017), P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels Sci Rep, 7(1):4872.
PMID: 28687781
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

H Kamata et al. (2017), KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis Sci Rep, 7(1):4556.
PMID: 28676645
Species: Human
Sample Types: Whole Cells
Applications: ICC

RCB Wong et al. (2017), Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors Stem Cell Res, 22(0):13-15.
PMID: 28952926
Species: Canine
Sample Types: Whole Tissue
Applications: IHC

Sofia Avnet (2016), Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-?B acivation Int. J. Cancer, 0(0):.
PMID: 27888521
Species: Human
Sample Types: Whole Cells
Applications: IHC

W Qin et al. (2016), Exosomes in Human Breast Milk Promote EMT Clin Cancer Res, 0(0):.
PMID: 27060153
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

(2016), The influence of warm ischemia elimination on kidney injury during transplantation - clinical and molecular study Sci Rep, 6(0):36118.
PMID: 27808277
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment Cancer Res., 0(0):.
PMID: 27864347
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

Jian-Yong Xu (2016), Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration Stem Cells, 0(0):.
PMID: 27333945
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Martinez L et al. (2015), CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients. PLoS ONE, 10(3):e0121421.
PMID: 25803686
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Riordan D et al. (2015), Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 10(7):e0128975.
PMID: 26176839
Species: Human
Sample Types: Whole Cells
Applications: ICC

Piao Y et al. (2014), Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors. Stem Cells Transl Med, 3(0):787-791.
PMID: 24904173
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Davaine J et al. (2014), Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. PLoS ONE, 9(9):e107642.
PMID: 25259713
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Liu T et al. (2014), Association between PON1 rs662 polymorphism and coronary artery disease. Eur J Clin Nutr, 68(9):1029-35.
PMID: 24918121
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Di Caro G et al. (2014), Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers. Clin Cancer Res, 20(8):2147-58.
PMID: 24523438
Species: Human
Sample Types: Whole Cells
Applications: ICC

Song S et al. (2013), Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells. Exp Cell Res, 319(8):1136-45.
PMID: 23458169
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

van der Veer E et al. (2013), Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype. Circ Res, 113(9):1065-75.
PMID: 23963726
Species: Human
Sample Types: Whole Cells
Applications: ICC

Milani C et al. (2013), Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture. BMC Cancer, 13(0):119.
PMID: 23497279
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Mohamed SA et al. (2012), Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve. Cardiol Res Pract, 2012(0):165957.
PMID: 22745920
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P

Ohshima S et al. (2010), Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging. J. Am. Coll. Cardiol., 55(12):1240-9.
PMID: 20298932
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Zhang F et al. (2010), Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts. Am. J. Physiol., Cell Physiol., 298(5):C1209-16.
PMID: 20200208
Species: Human
Sample Types: Whole Cells
Applications: ICC

Kim MH et al. (2010), The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices. Biomaterials, 31(6):1213-8.
PMID: 19914711
Species: Human
Sample Types: Whole Cells
Applications: ICC

Mohamed SA et al. (2010), Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve. Ann. Anat., 192(3):145-50.
PMID: 20427168
Species: Rat
Sample Types: Whole Cells
Applications: ICC

Zhou G et al. (2009), Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1. Am. J. Physiol. Lung Cell Mol. Physiol., 297(6):L1120-30.
PMID: 19801454
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Chetty A et al. (2008), Role of matrix metalloprotease-9 in hyperoxic injury in developing lung. Am. J. Physiol. Lung Cell Mol. Physiol., 295(4):L584-92.
PMID: 18658276
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC

Fujimoto S et al. (2008), Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy. J. Am. Coll. Cardiol., 52(23):1847-57.
PMID: 19038682
Species: Human
Sample Types: Whole Cells
Applications: ICC

Edward M et al. (2008), Gadodiamide contrast agent 'activates' fibroblasts: a possible cause of nephrogenic systemic fibrosis. J. Pathol., 214(5):584-93.
PMID: 18220317
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Hawsawi NM et al. (2008), Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes. Cancer Res., 68(8):2717-25.
PMID: 18413739
Species: Human
Sample Types: Whole Cells
Applications: ICC

Synenki L et al. (2007), Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation. Crit. Care Med., 35(3):842-8.
PMID: 17255860
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Hong KM et al. (2007), Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma. J. Biol. Chem., 282(31):22910-20.
PMID: 17556364
Species: Human
Sample Types: Whole Cells
Applications: ICC

Pan X et al. (0), Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes. Int J Med Sc12(3):248-55.
PMID: 25678842

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Customer Reviews (8)

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8 customer ratings

5 stars

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4 stars

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Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: WB

Sample Tested: Endothelial cells

Species: Mouse

Verified Customer | Posted 10/19/2021
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: IHC

Sample Tested: Testicular tissue

Species: Mouse

Verified Customer | Posted 08/10/2021
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: IHC

Sample Tested: Muscle tissue

Species: Mouse

Verified Customer | Posted 08/04/2021
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: IHC

Sample Tested: Breast cancer tissue

Species: Human

Verified Customer | Posted 02/19/2019
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: WB

Sample Tested: HEK293 human embryonic kidney cell line

Species: Human

Verified Customer | Posted 07/21/2018
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: HMVEC human microvascular endothelial cells

Species: Human

Verified Customer | Posted 12/28/2017
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: WB

Sample Tested: Skin tissue

Species: Mouse

Verified Customer | Posted 10/20/2017
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Name: Anonymous

Application: WB

Sample Tested: Lung tissue

Species: Mouse

Verified Customer | Posted 10/20/2017

The antibody works well for lung tissue of mouse.

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Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

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Complete Your Research

Complete Your Experiment

Applications

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody Scientific Data Examples

Western Blot

Western Blot

Immunocytochemistry

Immunohistochemistry

Immunohistochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Dual RNAscope ISH-IHC Compatible

Formulation, Preparation and Storage

Reconstitution Calculator

Background: alpha-Smooth Muscle Actin

Citations (60)

Customer Reviews (8)

Customer Images

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

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Product Datasheets

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