Human alpha-Smooth Muscle Actin APC-conjugated Antibody

Detection of alpha‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin APC-conjugated Monoclonal Antibody (Catalog # IC1420A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Mouse alpha-Smooth Muscle Actin by Immunohistochemistry

FGFR1 and TGF beta signaling activity in smooth muscle cells in a mouse atherosclerosis modelADissected mouse aorta demonstrating lipid‐rich plaques in brachiocephalic artery after 4 months of high‐fat diet compared to the normal diet in Apoe−/− mice. Panels b, d are cross sections of brachiocephalic artery from a, c stained with Oil Red O. L: lumen. Scale bar: 4 mm. n = 3 mice per group.BHistological analysis of mouse normal artery or atherosclerotic plaque in brachiocephalic artery with anti‐SM alpha‐actin, anti‐Notch3, and anti‐SM‐MHC antibodies. Nuclei were counterstained with DAPI (blue). L: lumen. Scale bar: 62 μm. n = 3 mice per group.C–FAnalysis of brachiocephalic artery of Apoe−/− mice maintained for 4 months on either normal or high‐fat diet using anti‐CD31 (green), anti‐p‐FGFR1 (red; C), anti‐FGFR1 (red; D), anti‐p‐Smad2 (red; E), and anti‐p‐Smad3 (red; F) antibodies. Nuclei were counterstained with DAPI (blue). Scale bar: 62 μm. L: lumen. M: media. n = 6 mice per group.G–JQuantification of the number of medial smooth muscle cells expressing p‐FGFR1, FGFR1, p‐Smad2, and p‐Smad3. ND: normal diet. HFD: high‐fat diet.Data information: All data shown as means ± SD (***P < 0.001 compared to ND, NS: not significant compared to ND; unpaired two‐tailed Student's t‐test). A full table of P‐values for this figure is shown in Appendix Table S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27189169), licensed under a CC-BY license. Not internally tested by R&D Systems.