Human alpha-Smooth Muscle Actin PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1420C
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
PerCP (Excitation = 482 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 1A4
Product Specifications
Immunogen
alpha-Smooth Muscle Actin N-terminal Synthetic Decapeptide
Specificity
Detects human alpha-Smooth Muscle Actin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human alpha-Smooth Muscle Actin PerCP-conjugated Antibody
Detection of alpha‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin PerCP-conjugated Monoclonal Antibody (Catalog # IC1420C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human alpha-Smooth Muscle Actin PerCP-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
References
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Alternate Names
AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA
Gene Symbol
ACTA2
Additional alpha-Smooth Muscle Actin Products
Product Documents for Human alpha-Smooth Muscle Actin PerCP-conjugated Antibody
Product Specific Notices for Human alpha-Smooth Muscle Actin PerCP-conjugated Antibody
For research use only
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