Skip to main content

Human/Mouse PON1 Antibody

Catalog # MAB4926 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Size / Price

Key Product Details

Species Reactivity

Human, Mouse


Western Blot



Antibody Source

Monoclonal Rat IgG2A Clone # 453223

Product Summary for Human/Mouse PON1 Antibody


E. coli-derived recombinant human PON1
Accession # P27169


Detects endogenous human and mouse PON1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON2 or rhPON3.







Scientific Data Images for Human/Mouse PON1 Antibody

Detection of Human/Mouse PON1 antibody by Western Blot.

Detection of Human/Mouse PON1 by Western Blot.

Western blot shows lysates of mouse liver tissue, human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse PON1 Monoclonal Antibody (Catalog # MAB4926) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse PON1 Antibody

Recommended Usage

Western Blot

0.5 µg/mL
Sample: Mouse liver tissue, human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PON1

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 1 (PON1, also known as serum paraoxonase) is a 355 amino acid, 43 kDa glycoprotein that is expressed in liver and is secreted into the bloodstream where it associates with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON1 is primarily a lactonase (EC that is thought to attenuate the oxidation of low-density lipoproteins (LDL). This may slow the initiation and progression of atherosclerosis. Human PON1 shares 83% and 81% aa identity with mouse and rat PON1, respectively.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Entrez Gene IDs

5444 (Human); 18979 (Mouse); 84024 (Rat)

Gene Symbol



Product Documents for Human/Mouse PON1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse PON1 Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Setting the standard in quality research reagents