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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 453223

Product Specifications

Immunogen

E. coli-derived recombinant human PON1
Ala30-Leu355
Accession # P27169

Specificity

Detects endogenous human and mouse PON1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON2 or rhPON3.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse PON1 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PON1

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 1 (PON1, also known as serum paraoxonase) is a 355 amino acid, 43 kDa glycoprotein that is expressed in liver and is secreted into the bloodstream where it associates with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON1 is primarily a lactonase (EC 3.1.8.1) that is thought to attenuate the oxidation of low-density lipoproteins (LDL). This may slow the initiation and progression of atherosclerosis. Human PON1 shares 83% and 81% aa identity with mouse and rat PON1, respectively.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Entrez Gene IDs

5444 (Human); 18979 (Mouse); 84024 (Rat)

Gene Symbol

PON1

UniProt

Additional PON1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse PON1 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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