MSK2/RSKB was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Phospho-MSK2/RSKB (S196) by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human HGF (Catalog # 294-HG) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat dorsal root ganglia and immersion fixed paraffin-embedded sections of human pancreas

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human HGF (Catalog #294-HG)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: MSK2

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name

Mitogen- and Stress-activated Protein Kinase 2

Alternate Names

RPS6KA4, RSKB

Entrez Gene IDs

8986 (Human); 56613 (Mouse)

Gene Symbol

RPS6KA4

Additional MSK2 Products

Product Documents for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody

For research use only

Citations
Reviews

Human/Mouse Phospho-MSK2/RSKB (S196) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF189

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MSK2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody

MSK2/RSKB in Human Pancreas.