Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human/Mouse Patched 1/PTCH(First Extracellular Loop) Antibody

Detection of Mouse Patched 1/PTCH by Western Blot.

Western blot shows lysates of MEF mouse embryonic feeder cells, Neuro-2A mouse neuroblastoma cell line, and mouse brain tissue. PVDF Membrane was probed with 3 µg/mL of Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Patched 1/PTCH at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Patched 1/PTCH(First Extracellular Loop) Antibody

Application

Recommended Usage

Western Blot

3 µg/mL
Sample: MEF mouse embryonic feeder cells, Neuro‑2A mouse neuroblastoma cell line, and mouse brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 1/PTCH

Patched homolog 1 (PTCH, also called PTC, PTC1 or PTCH1) is a 170‑175 kDa member of the Patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to PTC1, Smoothened is released and initiates downstream signaling. Mouse PTCH is a 12 transmembrane, 1434 aa glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for PTCH has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse PTCH shares 97% and 99% aa sequence identity with human and rat PTCH, respectively.

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Entrez Gene IDs

5727 (Human); 19206 (Mouse); 89830 (Rat)

Gene Symbol

PTCH1

UniProt

Q61115

Additional Patched 1/PTCH Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Patched 1/PTCH(First Extracellular Loop) Antibody

For research use only

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