Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB41051AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 413220
Product Specifications
Immunogen
E. coli-derived recombinant mouse Patched 1/PTCH
Specificity
Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Patched 1/PTCH
Patched homolog 1 (PTCH, also called PTC, PTC1 or PTCH1) is a 170‑175 kDa member of the Patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to PTC1, Smoothened is released and initiates downstream signaling. Mouse PTCH is a 12 transmembrane, 1434 aa glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for PTCH has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse PTCH shares 97% and 99% aa sequence identity with human and rat PTCH, respectively.
Alternate Names
BCNS, HPE7, mes, NBCCS, ptc1, PTCH1
Gene Symbol
PTCH1
UniProt
Additional Patched 1/PTCH Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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