Human/Mouse NCAM-1/CD56 Antibody

Name: Human/Mouse NCAM-1/CD56 Antibody
Brand: rnd
SKU: AF2408
Rating: 5 (2 reviews)

Catalog # AF2408

Citations (8)

(2)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human NCAM1/CD56 Leu20-Pro603 Accession # NP_001070150
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) ALCAM, rhBCAM and rhEpCAM is observed.

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Applications

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5-25 µg/mL	See below
Flow Cytometry	0.25 µg/10⁶ cells	Human peripheral blood mononuclear cells
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Item	Value
Knockout Validated	NCAM‑1/CD56 is specifically detected in U937 human histiocytic lymphoma parental cell line but is not detectable in NCAM‑1/CD56 knockout U937 human histiocytic lymphoma cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse NCAM-1/CD56 Antibody Scientific Data Examples

	Western Blot Detection of Human and Mouse NCAM‑1/CD56 by Western Blot. Western blot shows lysates of human brain (cerebellum and motor cortex) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NCAM-1/CD56 at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry NCAM-1/CD56 in SH-SY5Y Human Neuroblastoma Cells. SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 μM Retinoic Acid (0695/50) prior to immersion fixation. Neural Cell Adhesion Molecule 1 (NCAM-1)/CD56 was detected using a Goat Anti-Human/Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counter-stained with DAPI (blue). NCAM-1/CD56 immuno-reactivity was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry NCAM‑1/CD56 in BG01V Human Embryonic Stem Cells. NCAM-1/CD56 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
	Simple Western Detection of Human and Mouse NCAM‑1/CD56 by Simple Western^TM. Simple Western lane view shows lysates of human and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for NCAM-1/CD56 at approximately 143 kDa (as indicated) using 5 µg/mL for human lysates and 25 µg/mL for mouse lysates of Goat Anti-Human/Mouse NCAM-1/ CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Knockout Validated Western Blot Shows Human NCAM‑1/CD56 Specificity by Using Knockout Cell Line. Western blot shows lysates of U937 human histiocytic lymphoma cell line and human NCAM-1 knockout U937 human histiocytic lymphoma cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse NCAM‑1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for NCAM‑1/CD56 at approximately 160 kDa (as indicated) in the parental U937 human histiocytic lymphoma cell line, but is not detectable in knockout U937 human histiocytic lymphoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

Item	Value
References	Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273. Dickson, G. et al. (1987) Cell 50:1119. Lanier, L.L. et al. (1991) J. Immunol. 146:4421. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143. Doherty, P. et al. (1995) J. Neurobiol. 26:437. Eckardt, M. et al. (2000) J. Neurosci. 20:5234. Muller, D. et al. (1996) Neuron 17:413.
Long Name	Neural Cell Adhesion Molecule
Entrez Gene IDs	4684 (Human); 17967 (Mouse); 24586 (Rat)
Alternate Names	CD56, CD56 / NCAM-1, CD56 antigen, MSK39, N-CAM-1, NCAM-1, NCAM1, NCAMantigen recognized by monoclonal 5.1H11, neural cell adhesion molecule 1, neural cell adhesion molecule, NCAM

Citations for Human/Mouse NCAM-1/CD56 Antibody (8)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse NCAM-1/CD56 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

E Hassan et al. (2022), Abnormal ciliogenesis in decidual stromal cells in recurrent miscarriage Journal of reproductive immunology, 150(0):103486.
PMID: 35085989
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

C Merckx et al. (2022), Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-Transporters International Journal of Molecular Sciences, 23(6).
PMID: 35328671
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

W Kim et al. (2021), Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo The Journal of Biological Chemistry, 0(0):100372.
PMID: 33548223
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

A Yilmaz et al. (2020), Mapping Gene Circuits Essential for Germ Layer Differentiation via Loss-of-Function Screens in Haploid Human Embryonic Stem Cells Cell Stem Cell, 0(0).
PMID: 32735778
Species: Human
Sample Types: Whole Cells
Applications: ICC

E Slot et al. (2020), Generation of three iPSC lines from two patients with heterozygous FOXF1 mutations associated to Alveolar Capillary Dysplasia with Misalignment of the Pulmonary Veins Stem Cell Res, 44(0):101745.
PMID: 32169823
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

YP Xu et al. (2019), Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy J. Clin. Invest., 130(0).
PMID: 31310587
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Avior et al. (2017), Modeling Developmental and Tumorigenic Aspects of Trilateral Retinoblastoma via Human Embryonic Stem Cells Stem Cell Reports, 0(0).
PMID: 28392220
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Pistritto G et al. (2012), Divergent modulation of neuronal differentiation by caspase-2 and -9. PLoS ONE, 7(5):e36002.
PMID: 22629307

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Human/Mouse NCAM-1/CD56 Antibody

Name: Anonymous

Application: WB

Sample Tested: Adult brain (hippocampus),Brain olfactory membrane fraction,mouse brain

Species: Mouse

Verified Customer | Posted 04/15/2021

This antibody is working great without non-specific bands.
Human/Mouse NCAM-1/CD56 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Mouse brain cancer sample,Mouse brain cancer tissue

Species: Mouse

Verified Customer | Posted 01/19/2017

Immunohistochemistry-Floating section

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Human/Mouse NCAM-1/CD56 Antibody

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Key Product Details

Complete Your Research

Complete Your Experiment

Applications

Human/Mouse NCAM-1/CD56 Antibody Scientific Data Examples

Western Blot

Immunocytochemistry

Immunocytochemistry

Simple Western

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: NCAM-1/CD56

Citations for Human/Mouse NCAM-1/CD56 Antibody (8)

Customer Reviews (2)

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Human/Mouse NCAM-1/CD56 Antibody

Human/Mouse NCAM-1/CD56 Antibody

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