Human/Mouse NCAM‑1/CD56 Antibody
Catalog # AF2408 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human/Mouse NCAM‑1/CD56 Antibody
Immunogen
Leu20-Pro603
Accession # NP_001070150
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse NCAM‑1/CD56 Antibody
Detection of Human and Mouse NCAM‑1/CD56 by Western Blot.
Western blot shows lysates of human brain (cerebellum and motor cortex) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NCAM-1/CD56 at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.NCAM-1/CD56 in SH-SY5Y Human Neuroblastoma Cells.
SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 μM Retinoic Acid (0695/50) prior to immersion fixation. Neural Cell Adhesion Molecule 1 (NCAM-1)/CD56 was detected using a Goat Anti-Human/Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counter-stained with DAPI (blue). NCAM-1/CD56 immuno-reactivity was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.NCAM‑1/CD56 in BG01V Human Embryonic Stem Cells.
NCAM-1/CD56 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human/Mouse NCAM‑1/CD56 Antibody
CyTOF-ready
Flow Cytometry
Sample: Human peripheral blood mononuclear cells
Immunocytochemistry
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line and BG01V human embryonic stem cells differentiated into neural progenitor cells
Knockout Validated
Simple Western
Sample: Human and mouse brain (cerebellum) tissue
Western Blot
Sample: Human brain (cerebellum and motor cortex) tissue and mouse brain (cerebellum) tissue
Reviewed Applications
Read 2 reviews rated 5 using AF2408 in the following applications:
Formulation, Preparation and Storage
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Reconstitution
Formulation
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Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NCAM-1/CD56
Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).
References
- Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
- Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
- Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
- Dickson, G. et al. (1987) Cell 50:1119.
- Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
- Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
- Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
- Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
- Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
- Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
- Doherty, P. et al. (1995) J. Neurobiol. 26:437.
- Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
- Muller, D. et al. (1996) Neuron 17:413.
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Alternate Names
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UniProt
Product Documents for Human/Mouse NCAM‑1/CD56 Antibody
Product Specific Notices for Human/Mouse NCAM‑1/CD56 Antibody
For research use only
Citations for Human/Mouse NCAM‑1/CD56 Antibody (13)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse NCAM‑1/CD56 Antibody include:
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Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: Flow Cytometry,IHCB Krämer et al. (2023-01-01), Single-cell RNA sequencing identifies a population of human liver-type ILC1s Cell Reports, 2023-042(1):111937.
PMID: 36640314 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCH Tomita et al. (2022-09-16), 7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome Elife, 2022-011(0).
PMID: 36111785 -
Species: Human,Mouse
Sample Types: Cell Lysates
Applications: Western BlotHA Taylor et al. (2022-04-20), PTPRD and DCC Are Novel BACE1 Substrates Differentially Expressed in Alzheimer's Disease: A Data Mining and Bioinformatics Study International Journal of Molecular Sciences, 2022-023(9).
PMID: 35562959 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCHW Cheng et al. (2022-04-19), Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 2022-013(1):2027.
PMID: 35440118 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCC Merckx et al. (2022-03-17), Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-Transporters International Journal of Molecular Sciences, 2022-023(6).
PMID: 35328671 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCE Hassan et al. (2022-01-19), Abnormal ciliogenesis in decidual stromal cells in recurrent miscarriage Journal of reproductive immunology, 2022-0150(0):103486.
PMID: 35085989 -
Species: Human
Sample Types: Whole Tissues
Applications: IHCDA Clark et al. (2022-01-14), CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells Journal of reproductive immunology, 2022-0150(0):103477.
PMID: 35051828 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotW Kim et al. (2021-02-03), Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo The Journal of Biological Chemistry, 2021-00(0):100372.
PMID: 33548223 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Yilmaz et al. (2020-07-30), Mapping Gene Circuits Essential for Germ Layer Differentiation via Loss-of-Function Screens in Haploid Human Embryonic Stem Cells Cell Stem Cell, 2020-00(0).
PMID: 32735778 -
Species: Human
Sample Types: Whole Cells
Applications: ICCE Slot et al. (2020-03-04), Generation of three iPSC lines from two patients with heterozygous FOXF1 mutations associated to Alveolar Capillary Dysplasia with Misalignment of the Pulmonary Veins Stem Cell Res, 2020-044(0):101745.
PMID: 32169823 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PYP Xu et al. (2019-07-16), Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy J. Clin. Invest., 2019-0130(0).
PMID: 31310587 -
Species: Human
Sample Types: Whole Cells
Applications: ICCY Avior et al. (2017-04-06), Modeling Developmental and Tumorigenic Aspects of Trilateral Retinoblastoma via Human Embryonic Stem Cells Stem Cell Reports, 2017-00(0).
PMID: 28392220 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotPistritto G et al. (2012-05-18), Divergent modulation of neuronal differentiation by caspase-2 and -9. PLoS ONE, 2012-07(5):e36002.
PMID: 22629307
There are no citations that match your criteria.
Customer Reviews for Human/Mouse NCAM‑1/CD56 Antibody (2)
Customer Images
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Application: Western BlotSample Tested: mouse brain, Brain olfactory membrane fraction and Adult brain (hippocampus)Species: MouseVerified Customer | Posted 04/15/2021This antibody is working great without non-specific bands.
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Application: ImmunohistochemistrySample Tested: Mouse brain cancer sample and Mouse brain cancer tissueSpecies: MouseVerified Customer | Posted 01/19/2017Immunohistochemistry-Floating section
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