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Human/Mouse GATA-3 Antibody
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Species Reactivity: | Human, Mouse |
| Immunogen: |
E. coli-derived recombinant human GATA-3 Pro135-Ser258 Accession # P23771 |
| Applications: | Dual RNAscope ISH-IHC, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2B Clone # 634913 |
| Specificity: |
Detects human and mouse GATA-3 in Western blots. |
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Western BlotDetection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Moue Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for full length (FL) GATA-3 at approximately 52 kDa and the splice form (SF) found in MCF-7 cells at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunocytochemistryGATA‑3 in MCF‑7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunohistochemistryGATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Simple WesternDetection of Human GATA‑3 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for GATA‑3 at approximately 53 (splice variant) and 61 kDa (full length) as indicated, using 10 µg/mL of Mouse Anti-Human/Mouse GATA‑3 Monoclonal Antibody (Catalog # MAB6330). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
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Dual RNAscope ISH-IHC CompatibleGATA-3 in Human Breast Cancer Tissue Using Dual RNAscope® ISH and IHC. GATA-3 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 403551) followed by immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog# MAB6330) at 5ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001). Tissue was stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (Catalog # 322500). |
Citations for Human/Mouse GATA-3 Antibody (9)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse GATA-3 Antibody include:
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Species: Human
Sample Types: Synovial Fluid
Applications: Western BlotF Caso et al. (2022-01-01), Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation Autoimmunity reviews, 21(12):103207.
PMID: 36191778 -
Species: Human
Sample Types: Spheroids
Applications: IHCY Fan et al. (2021-01-01), Generation of human blastocyst-like structures from pluripotent stem cells Cell Discovery, 7(1):81.
PMID: 34489415 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Li et al. (2021-01-01), Predictive role of endometrial T-bet/GATA3 ratio during mid-luteal phase for live birth in patients undergoing in vitro fertilization: A retrospective observational study Journal of reproductive immunology, 149(0):103465.
PMID: 34953324 -
Species: Mouse
Sample Types: Embryo
Applications: IHCPT Ruane et al. (2020-01-01), Protein O-GlcNAcylation Promotes Trophoblast Differentiation at Implantation Cells, 9(10).
PMID: 33036308 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PME Kadin et al. (2018-01-01), IL-13 is produced by tumor cells in Breast Implant Associated Anaplastic Large Cell Lymphoma: Implications for pathogenesis Hum. Pathol., 0(0).
PMID: 29689246 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotP Shahi et al. (2017-01-01), ZNF503/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28258171 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometrySC Hall et al. (2017-01-01), Increased TREM-2 expression on the subsets of CD11c(+) cells in the lungs and lymph nodes during allergic airway inflammation Sci Rep, 7(1):11853.
PMID: 28928485 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PJoseph Klausner (2016-01-01), Analysis of histological and immunological parameters of metastatic lymph nodes from colon cancer patients reveals that T-helper 1 type immune response is associated with improved overall survival Medicine (Baltimore), 95(45):e5340.
PMID: 27828856 -
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIPHosokawa H et al. (2015-01-01), Methylation of Gata3 protein at Arg-261 regulates transactivation of the Il5 gene in T helper 2 cells. J Biol Chem, 290(21):13095-103.
PMID: 25861992
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Customer Reviews for Human/Mouse GATA-3 Antibody (1)
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Application: ICC/IFSample Tested: BeWo human choriocarcinoma cell lineSpecies: HumanVerified Customer | Posted 03/20/2023BeWo Cell. Gata3 & Merge with DAPI.
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