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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 685641

Product Summary for Human MMR/CD206 APC-conjugated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897

Specificity

Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse MMR is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human MMR/CD206 APC-conjugated Antibody

Detection of MMR/CD206 antibody in Human Immature Dendritic Cells antibody by Flow Cytometry.

Detection of MMR/CD206 in Human Immature Dendritic Cells by Flow Cytometry.

Human monocyte-derived immature dendritic cells were stained with Mouse Anti-Human MMR/CD206 APC-conjugated Monoclonal Antibody (Catalog # FAB25342A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MMR/CD206 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human monocyte-derived immature dendritic cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using FAB25342A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin‑coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Gene Symbol

MRC1

UniProt

Additional MMR/CD206 Products

Product Documents for Human MMR/CD206 APC-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MMR/CD206 APC-conjugated Antibody

For research use only

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