Human MMP-14/MT1-MMP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB918
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5H2
Product Specifications
Immunogen
Human MMP-14 synthetic peptide
AYIREGHEKQA
Accession # P50281
AYIREGHEKQA
Accession # P50281
Specificity
Detects recombinant human (rh) MMP-14 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -7, -8, -9, -10, -12, -13 or the catalytic domains of rhMMP-15 and -16.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MMP-14/MT1-MMP Antibody
Detection of Human MMP‑14/MT1‑MMP by Western Blot.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP-14 in Human Benign Nodular Hyperplasia.
MMP-14 was detected in immersion fixed paraffin-embedded sections of human benign nodular hyperplasia using 25 µg/mL Mouse Anti-Human MMP-14 Monoclonal Antibody (Catalog # MAB918) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MMP-14/MT1-MMP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human benign nodular hyperplasia
Sample: Immersion fixed paraffin-embedded sections of human benign nodular hyperplasia
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑14 (Catalog # 918‑MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑14 (Catalog # 918‑MP), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Human placenta tissue
Sample: Human placenta tissue
Reviewed Applications
Read 1 review rated 4 using MAB918 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-14/MT1-MMP
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and Integrin alphaV (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2).
References
- Seike, M. (2003) Cancer Lett. 194:1.
- Sato, H. et al. (1994) Nature 370:61.
Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Alternate Names
MMP-X1, MMP14, MT-MMP1, MT1-MMP
Entrez Gene IDs
4323 (Human)
Gene Symbol
MMP14
UniProt
Additional MMP-14/MT1-MMP Products
Product Documents for Human MMP-14/MT1-MMP Antibody
Product Specific Notices for Human MMP-14/MT1-MMP Antibody
For research use only
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