Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK3 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Shows Human MEKK3 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MEKK3 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MEKK3 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of MEKK3 by Western Blot

MEKK3 is essential for embryonic vascular development and neonatal brain vascular integrity.(i) Immunoblots show expression of VEGFR2, MEKK3 and T18/S19 phosphorylated MLC2 (MLC2pT18/S19) in freshly isolated BMEC from three Mekk3NCL and three Mekk3 iEC−/− littermate pups as indicated. GAPDH expression is shown as loading controls. Elevated MLC2pT18/S19 was observed in three independently isolated pairs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26235885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MEKK3 Antibody

Application

Recommended Usage

Knockout Validated

MEKK3 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in MEKK3 knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line

Reviewed Applications

Read 4 reviews rated 4 using MAB6095 in the following applications:

ELISA (1 Review)
Microarray (1 Review)
Microarrays (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEKK3

MAPK/ERK kinase kinase 3 (MEKK3), also known as MEK kinase 3 and MAP3K3, is a widely expressed 71 kDa Ser/Thr kinase in the SEK, MEK, and MyD88‑IRAK‑TRAF6 signal transduction pathways. It contains a PB1 domain (aa 45‑123), a region with several phosphorylation sites, and a protein kinase domain (aa 361‑621). MEKK3 is involved in tissue morphogenesis, inflammation, and the response to cellular stress. Its activation leads to the activation of NF kappaB following stimulation of IL-1 R, TLR4, TLR8, TNF RI, and the AT1 angiotensin receptor.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 3

Alternate Names

MAP3K3, MAPKKK3

Entrez Gene IDs

4215 (Human); 26406 (Mouse); 303604 (Rat)

Gene Symbol

MAP3K3

UniProt

Q99759 (Human)

Additional MEKK3 Products

Product Documents for Human MEKK3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MEKK3 Antibody

For research use only

Citations
Reviews

Human MEKK3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6095

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all MEKK3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MEKK3 Antibody

Detection of Human MEKK3 by Western Blot.