Human MCM4 Antibody

Detection of Human MCM4 by Western Blot.

Western Blot shows lysates of HCT-116 human colorectal carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human MCM4 Monoclonal Antibody (Catalog # MAB11718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCM4 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human MCM4 by Simple Western^TM.

Left: Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.1 mg/ml. A specific band was detected for MCM4 at approximately 107 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human MCM4 Monoclonal Antibody (Catalog # MAB11718) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MCM4 Monoclonal Antibody (Catalog # MAB11718) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the Jurkat human acute T cell leukemia cell line.

Detection of MCM4 in Human Cervical Cancer.

MCM4 was detected in immersion fixed paraffin-embedded sections of human cervical cancer using Mouse Anti-Human MCM4 Monoclonal Antibody (Catalog # MAB11718) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.