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Human MAP3K8/Tpl2/COT Antibody
Catalog # MAB4586 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 634130
Product Summary for Human MAP3K8/Tpl2/COT Antibody
Immunogen
E. coli-derived recombinant human MAP3K8/Tpl2/COT
Gln173-Gln395
Accession # P41279
Gln173-Gln395
Accession # P41279
Specificity
Detects human MAP3K8/Tpl2/COT in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MAP3K8/Tpl2/COT Antibody
Detection of Human MAP3K8/Tpl2/COT by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Human MAP3K8/Tpl2/COT Monoclonal Antibody (Catalog # MAB4586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for MAP3K8/Tpl2/COT at approximately 58 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MAP3K8/Tpl2/COT Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line
Sample: Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 1 publication using MAB4586 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAP3K8/Tpl2/COT
MAP3K8, also called Tpl2 (tumor progression locus 2) or COT (Cancer Osaka Thyroid oncogene) is a cytosolic Ser/Thr kinase. MAP3K8 is one of the MAP kinase kinase (MAPKK) kinases that regulates the ERK1/ERK2 pathway in response to IL-1, facilitating IL-8 and MIP-1 beta production. MAP3K8 activates I kappaB kinases and induces the nuclear production of NF-kappa B. Human MAP3K8 shares 97% and 98% amino acid (aa) identity with mouse and rat MAP3K8, respectively, within the region used as an immunogen. Alternative start sites can give a 58 kDa (467 aa) isoform with stronger activity but shorter half-life than a 52 kDa (397 aa) isoform.
Product Documents for Human MAP3K8/Tpl2/COT Antibody
Product Specific Notices for Human MAP3K8/Tpl2/COT Antibody
For research use only
Citations for Human MAP3K8/Tpl2/COT Antibody (1)
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Citations are publications that use Bio-Techne products. Selected citations for Human MAP3K8/Tpl2/COT Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotBD Lehmann et al. (2019-06-11), Identification of targetable recurrent MAP3K8 rearrangements in melanomas lacking known driver mutations Mol. Cancer Res., 2019-00(0).
PMID: 31186280
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