Human LAP (TGF-beta 1) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB246
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Complex Species Category, Virus - HIV-1
Applications
Validated:
Neutralization, Immunocytochemistry
Cited:
Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Multiplex Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 27235
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Specificity
Detects human LAP in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human LAP (TGF-beta 1) Antibody
LAP (TGF-beta 1) in HEK293 Human Cell Line.
LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LAP TGF-beta 1 Inhibition of TGF-beta 1 Activity and Neutralization by Human LAP TGF-beta 1 Antibody.
Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND50 is typically 1-5 µg/mL.
Detection of LAP (TGF-beta 1) by Western Blot
Specificity of R58 and L59 antibodies. (a) Schematic structure of a GST- rhLTGF-beta 1 protein generated in bacteria and a commercial rhLAP beta1 generated in insect cells. The PLK cleavage site is indicated by arrowhead. (b-e) GST-LTGF-beta 1/rhLAP beta1was incubated with/without PLK or PLN for 45 min at 37°C, and thereafter equal amounts of samples containing either 50 ng GST-rhLTGF-beta 1 or 500 ng rhLAP beta1 were subjected to each lane in SDS-PAGE under reducing conditions followed by Western blot analyses using R58/L59 antibodies (b, d, respectively). Membranes used in panels b and d were reprobed with monoclonal (c) or polyclonal (e) anti-LAP antibodies. Lanes 1, intact GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 2, PLK digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 3, PLK; lane 4, PLN digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lane 5, PLN. Representative results from three independent experiments with a similar result are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24877031), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human LAP (TGF-beta 1) Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Neutralization
Measured by its ability to neutralize LAP TGF‑ beta1 inhibition of TGF‑ beta1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta1 and 1 ng/mL Recombinant Human TGF‑ beta1.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAP (TGF-beta 1)
Long Name
Latency-associated Peptide
Alternate Names
LAP (TGFbeta 1)
Entrez Gene IDs
7040 (Human)
Gene Symbol
TGFB1
Additional LAP (TGF-beta 1) Products
Product Documents for Human LAP (TGF-beta 1) Antibody
Product Specific Notices for Human LAP (TGF-beta 1) Antibody
For research use only
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