Western blot shows nuclear extracts of LNCaP human prostate cancer cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4049) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IRF2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human IRF2 by Simple Western^TM.

Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IRF2 at approximately 61 kDa (as indicated) using 5 µg/mL of Goat Anti-Human IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4049) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IRF2 Antibody

Application

Recommended Usage

Simple Western

5 µg/mL
Sample: U937 human histiocytic lymphoma cell line

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line chronic myelogenous leukemia, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF2

Interferon Regulatory Factor 2 (IRF2) belong to the interferon regulatory transcription factor family. IRF2 functions as a regulator of type I interferons influencing cellular proliferation and immune response through transcriptional regulation. IRF2 competitively inhibits IRF1 gene activation, as well as, stimulating transcription on its own. Over-expression of IRF2 in NIH3T3 cells leads to transformation, whereas, loss of IRF2 expression in knock out mice leads to a variety of abnormalities including loss of NK cells due to increased apoptosis.

Long Name

Interferon Regulatory Factor 2

Alternate Names

DKFZp686F0244, interferon regulatory factor 2, IRF-2

Entrez Gene IDs

3660 (Human); 16363 (Mouse); 290749 (Rat)

Gene Symbol

IRF2

UniProt

P14316

Additional IRF2 Products

Product Documents for Human IRF2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IRF2 Antibody

For research use only

Citations
Reviews

Human IRF2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4049

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IRF2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IRF2 Antibody

Detection of Human IRF2 by Western Blot.

Detection of Human IRF2 by Simple WesternTM.