Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Integrin beta1/CD29 in MG‑63 Human Cell Line.

Integrin beta1/CD29 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human Integrin beta1/CD29 Monoclonal Anti-body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti-body (yellow; NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Integrin beta1/CD29 in A549 Cells.

Integrin beta1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB1778) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

Applications for Human Integrin beta1/CD29 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line, A549 human lung carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue

Immunoprecipitation

Marshall, J.F. et al. (1998) Br. J. Cancer 77:522.

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin beta1/CD29 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits (1).

References

Wayner, E.A. and W.G. Carter (1987) J. Cell Biol. 105:1873.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Human Integrin beta1/CD29 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin beta1/CD29 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin beta1/CD29 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1778

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin beta1/CD29 Antibody

Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.