H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Integrin alpha8 in H4 Human Cell Line.

Integrin a8 was detected in immersion fixed H4 human neuroglioma cell line using Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Integrin alpha 8 by Immunocytochemistry/ Immunofluorescence

Integrin-based subcellular adhesion structures on RGD and LDVP surfaces.Fab (100 nM for HP1/7 and 20 nM for the others) and Sir-actin (10 nM) were added 10 min before live-cell imaging of cells seeded for 1 h. a Integrin alphaV (AF488-13C2 Fab, green in the merged images) and actin stress fibers (SiR-actin, red in the merged images) on RGD-54pN. Intensity profiles (bottom right panel) are along the dashed line in the third enlarged image. Arbitrary units (a.u.). b Integrin alpha4 (AF488-HP1/7 Fab) and actin stress fibers (SiR-actin) on LDVP-54pN. c Integrin alpha4 (Cy3-HP1/7 Fab) and alphaV (AF647-13C2 Fab). d Paxillin (AF488, green) immunostaining of fixed cells imaged with SiR-actin (red) and alphaV (Cy3-13C2 Fab) or alpha4 (Cy3-HP1/7 Fab). e Extended open beta1 (Cy3-12G10 Fab) and closed beta1 (AF647-mAb13 Fab) on RGD-54pN. f Extended open beta1 (Cy3-12G10 Fab) and closed beta1 (AF647-mAb13 Fab) on LDVP-54pN. Boxed regions are magnified in the bottom row or right column. Scale bars: 10 µm (white), 2 µm (yellow). Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36463259), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Integrin alpha8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: H4 human neuroglioma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed H4 human neuroglioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 8

Integrin alpha8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta1. Integrin alpha8/ beta1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta1 and Nephronectin. It is principally expressed in smooth muscle cells. Human alpha8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha8 shares 90% aa sequence identity with mouse alpha8 protein.

Alternate Names

ITGA8

Entrez Gene IDs

8516 (Human); 241226 (Mouse); 84381 (Rat)

Gene Symbol

ITGA8

UniProt

P53708

Additional Integrin alpha 8 Products

Product Documents for Human Integrin alpha8 Antibody

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Product Specific Notices for Human Integrin alpha8 Antibody

For research use only

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Flow Cytometry

Human Integrin alpha8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6194

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Scientific Data Images for Human Integrin alpha8 Antibody

Detection of Integrin alpha8 in H4 Human Cell Line by Flow Cytometry.