Human IL-7R alpha/CD127 PE-conjugated Antibody

Name: Human IL-7R alpha/CD127 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB306P
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB306P

Citations (2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha Extracellular domain
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 40131
Specificity:	Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL‑10 R, rhIL‑2 R beta, rhIL‑5 R alpha, and rhIL‑6 R.

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Applications for Human IL-7R alpha/CD127 PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-7R alpha/CD127 PE-conjugated Antibody

	Flow Cytometry Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IL-7 Ra/CD127 PE-conjugated Monoclonal Antibody (Catalog # FAB306P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-/CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

Item	Value
References	Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193. Goodwin, R.G. et al. (1990) Cell 60:941. Noguchi, M. et al. (1993) Science 262:1877. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329. Park, L.S. et al. (2000) J. Exp. Med. 192:659. Pandey, A. et al. (2000) Nat. Immunol. 1:59. Reche, P.A. et al. (2001) J. Immunol. 167:336. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159. Jahn, T. et al. (2007) Blood 110:1840. Lai, L. et al. (2006) Blood 107:1776.
Long Name	Interleukin 7 Receptor alpha
Entrez Gene IDs	3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names	CD127, CD127 antigen, CD127ILRA, CDW127, IL-7 R alpha, IL-7 receptor subunit alpha, IL-7R alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7Ra, IL7R, IL7R alpha, IL7RA, interleukin 7 receptor, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, interleukin-7 receptor subunit alpha

Citations for Human IL-7R alpha/CD127 PE-conjugated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-7R alpha/CD127 PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Pandya AD et al. (2011), Identification of human NK17/NK1 cells. PLoS ONE, 6(10):e26780.
PMID: 22039549
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Fluur C et al. (2007), Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection. J. Immunol., 178(8):5340-50.
PMID: 17404319

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Customer Reviews for Human IL-7R alpha/CD127 PE-conjugated Antibody (1)

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Human IL-7 R alpha /CD127 PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood cells

Species: Human

Verified Customer | Posted 07/07/2017

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Product Documents for Human IL-7R alpha/CD127 PE-conjugated Antibody

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Human IL-7R alpha/CD127 PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-7R alpha/CD127 PE-conjugated Antibody

Scientific Data Examples for Human IL-7R alpha/CD127 PE-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IL-7R alpha/CD127

Citations for Human IL-7R alpha/CD127 PE-conjugated Antibody (2)

Customer Reviews for Human IL-7R alpha/CD127 PE-conjugated Antibody (1)

Human IL-7 R alpha /CD127 PE-conjugated Antibody

Product Documents for Human IL-7R alpha/CD127 PE-conjugated Antibody

Product Datasheets

COA

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