Detects human IL-33 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human IL-33 and mature recombinant mouse IL-33 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-33 Propeptide Antibody

Detection of Human IL-33 Propeptide by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-33 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-33 Propeptide at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

IL‑33 in HUVEC Human Cells.

IL-33 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human IL-33 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4810) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human IL-33 Propeptide Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33 (interleukin 33; also NF-HEV and IL-1F11) is a 31-32 kDa member of the IL-1 family of proteins. It is expressed in endothelial cells and reportedly has two functions; first, it induces Th2-type cytokines, and second, it acts as a nuclear transcription factor. Human IL-33 is 270 amino acids (aa) in length. It contains a putative proregion (aa 1-111) and a mature segment (aa 112-270). The proregion contains a bipartite NLS (aa 61-78), plus an alpha-helical homeodomain-like HTH motif (aa 1-65). The HTH motif mediates nuclear localization and heterochromatin association. A potential caspase 1 cleavage site exists in human IL-33 (Ser111-Ser112), but not in IL-33 from canine, porcine or bovine. There is one splice variant that shows a deletion of aa 113-118. Over aa 1-111, human IL-33 propeptide is 45% aa identical to mouse IL-33 propeptide.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse)

Gene Symbol

IL33

UniProt

O95760

Additional IL-33 Products

Product Documents for Human IL-33 Propeptide Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-33 Propeptide Antibody

For research use only

Citations
Reviews

Human IL-33 Propeptide Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4810

Key Product Details

Species Reactivity

Validated:

Cited:

Applications