Human IL-21R PE-conjugated Antibody

Name: Human IL-21R PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB9911P

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB9911P

Citations (6)

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Clone 152512 was used by HLDA to establish CD designation

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Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R Cys20-Pro236 Accession # Q9HBE5
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 152512
Specificity:	Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh)IL‑2 R beta or rmIL‑21 R.

View Full Technical Details for Human IL-21R PE-conjugated Antibody

Applications for Human IL-21R PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-21R PE-conjugated Antibody

	Flow Cytometry Detection of IL-21 R in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL-21 R PE-conjugated Monoclonal Antibody (Catalog # FAB9911P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

Item	Value
References	Parrish-Novak, et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name	Interleukin 21 Receptor
Entrez Gene IDs	50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names	CD360, CD360 antigen, IL-21 R, IL-21 receptor, IL-21R, IL21R, MGC10967, NILR, Novel interleukin receptor, interleukin 21 receptor, interleukin-21 receptor

Citations for Human IL-21R PE-conjugated Antibody (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-21R PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Llinas L et al. (2011), Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 134(2):113-21.
PMID: 20951740
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Sarra M et al. (2011), IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia. J. Immunol., 186(9):5435-42.
PMID: 21441456
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Liu Y et al. (2011), Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription. Immunology, 132(4):540-8.
PMID: 21214545
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

de Totero D et al. (2010), Heterogeneous expression and function of IL-21R and susceptibility to IL-21-mediated apoptosis in follicular lymphoma cells. Exp. Hematol., 38(5):373-83.
PMID: 20193734
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lee WW et al. (2010), Regulating human Th17 cells via differential expression of IL-1 receptor. Blood, 115(3):530-40.
PMID: 19965648
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Perez S et al. (0), Effect of IL-21 on NK cells derived from different umbilical cord blood populations. Int Immuno18(1):49-58.
PMID: 16291655

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Showing 1 - 6 of 6 citations Showing All

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Human IL-21R PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-21R PE-conjugated Antibody

Scientific Data Examples for Human IL-21R PE-conjugated Antibody

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