Human IL-21R Antibody

Name: Human IL-21R Antibody
Brand: R&D Systems
SKU: MAB9911
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB9911

Citations (3)

(1)

Product Datasheet / COA / SDS

Clone 152512 was used by HLDA to establish CD designation

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R Cys20-Pro236 Accession # Q9HBE5
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 152512
Specificity:	Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh)IL‑2 R beta or rmIL‑21 R.

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Applications for Human IL-21R Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Human whole blood CD19⁺ B cells
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

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Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

Item	Value
References	Parrish-Novak, et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name	Interleukin 21 Receptor
Entrez Gene IDs	50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names	CD360, CD360 antigen, IL-21 R, IL-21 receptor, IL-21R, IL21R, MGC10967, NILR, Novel interleukin receptor, interleukin 21 receptor, interleukin-21 receptor

Citations for Human IL-21R Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-21R Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Cabezon R et al. (2011), Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 134(2):167-73.
PMID: 20933012
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Matesanz-Isabel J et al. (2011), New B-cell CD molecules. Immunol. Lett., 134(2):104-12.
PMID: 20933010
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ansen S et al. (2008), Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21. Clin. Cancer Res., 14(19):6125-36.
PMID: 18829491

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Human IL-21R Antibody

Name: Anonymous

Application: CyTOF

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 05/04/2022

Gated on CD45+ PBMCs

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Product Documents for Human IL-21R Antibody

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Human IL-21R Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-21R Antibody

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IL-21R

Citations for Human IL-21R Antibody (3)

Customer Reviews for Human IL-21R Antibody (1)

Customer Images

Human IL-21R Antibody

Product Documents for Human IL-21R Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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