Human IL-1 alpha/IL-1F1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF200
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry
Cited:
Electrochemiluminescent Assay, ELISA Development, ELISA Microarray Development, Functional Assay, Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IL-1 alpha/IL-1F1 (R&D Systems, Catalog # 200-LA)
Ser113-Ala271
Accession # Q53QF9
Ser113-Ala271
Accession # Q53QF9
Specificity
Detects human IL-1 alpha/IL-1F1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse IL‑1 alpha, recombinant porcine IL‑1 alpha, recombinant rat IL‑1 alpha, recombinant human (rh) IL-1 sRI, and rhIL-1 sRII is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IL-1 alpha/IL-1F1 Biotinylated Antibody
IL‑1 alpha/IL‑1F1 in Human PBMCs.
IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human IL-1a/IL-1F1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF200) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL‑1 alpha/IL‑1F1 in Human PBMCs.
IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human IL-1a/IL-1F1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF200) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Human IL-1 alpha / IL-1F1 ELISA Standard Curve
Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) was serially diluted and captured by Mouse Anti-Human IL-1 alpha/IL-1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human IL-1 alpha/IL-1F1 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensin
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensin
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA)
Sample: Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA)
Human IL-1 alpha/IL-1F1 Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using BAF200 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 alpha/IL-1F1
IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Entrez Gene IDs
Gene Symbol
IL1A
UniProt
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Human IL-1 alpha/IL-1F1 Biotinylated Antibody
Product Specific Notices for Human IL-1 alpha/IL-1F1 Biotinylated Antibody
For research use only
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