Human IL-18 R alpha /IL-1 R5 Antibody

Name: Human IL-18 R alpha /IL-1 R5 Antibody
Brand: R&D Systems
SKU: AF840

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF840

Citations (5)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5 Glu20-Arg329 Accession # Q13478
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.

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Applications for Human IL-18 R alpha /IL-1 R5 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR)
Flow Cytometry	2.5 µg/10⁶ cells	Human CD3⁺ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Item	Value
Neutralization	Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-18 R alpha /IL-1 R5 Antibody

	Immunohistochemistry IL‑18 R alpha /IL‑1 R5 in Human Skin. IL‑18 Ra/IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Neutralization IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R alpha /IL‑1 R5 Antibody. Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50C). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND₅₀ is typically 0.1-0.4 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.

Item	Value
References	Parnet, P. et al. (1996) J. Biol. Chem. 271:3967. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Alternate Names	CD218a, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Citations for Human IL-18 R alpha /IL-1 R5 Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-18 R alpha /IL-1 R5 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

A Gibbs et al. (2016), MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation Mucosal Immunol, 0(0).
PMID: 27049062
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016), Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 6(0):26757.
PMID: 27225603
Species: Human
Sample Types: Whole Cells
Applications: ICC

Dusseaux M et al. (2011), Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood, 117(4):1250-9.
PMID: 21084709
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sallmon H et al. (2010), Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system. J. Interferon Cytokine Res., 30(2):55-8.
PMID: 20028206
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

Felderhoff-Mueser U et al. (2005), Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain. Ann. Neurol., 57(1):50-9.
PMID: 15622543

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Human IL-18 R alpha /IL-1 R5 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-18 R alpha /IL-1 R5 Antibody

Scientific Data Examples for Human IL-18 R alpha /IL-1 R5 Antibody

Immunohistochemistry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-18 R alpha/IL-1 R5

Citations for Human IL-18 R alpha /IL-1 R5 Antibody (5)

Product Documents for Human IL-18 R alpha /IL-1 R5 Antibody

Product Datasheets

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