Human IL-18/IL-1F4 Antibody

Name: Human IL-18/IL-1F4 Antibody
Brand: R&D Systems
SKU: MAB91244

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB91244

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-18/IL-1F4 Tyr37-Asp193 Accession # Q14116
Applications:	ELISA
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 1072H
Specificity:	Detects human IL-18/IL-1F4 in direct ELISAs. In a sandwich immunoassay, with Goat Anti-Human IL-18 BPa Antigen Affinity Purified Polyclonal Antibody (Catalog # AF119) the human IL-18/IL-18 BPa complex is detected.

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Applications for Human IL-18/IL-1F4 Antibody

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). This antibody pair detects the human IL-18/IL-18 BPa complex.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-18/IL-18 BPa Complex DuoSet ELISA Kit (Catalog # ) for convenient development of a sandwich ELISA or the Human IL-18 BPa Quantikine ELISA Kit (Catalog # ) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-18/IL-1F4 Antibody

	ELISA Human IL‑18/IL-18 BPa Complex ELISA Standard Curve. Recombinant Human IL-18/IL-18 BPa complex protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed
IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8⁺ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4⁺ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4⁺ T cells (11).
IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

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References	Dinarello, C.A. et al. (2013) Front. Immunol. 4:289. Smith, D.E. (2011) J. Leukoc. Biol. 89:383. Gu, Y. et al. (1997) Science 275:206. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Cheung, H. et al. (2005) J. Immunol. 174:5351. Novick, D. et al. (1999) Immunity 10:127. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769. Lalor, S.J. et al. (2011) J. Immunol. 186:5738. Li, J. et al. (2004) Cell. Immunol. 227:103. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665. Ushio, S. et al. (1996) J. Immunol. 156:4274. Gaggero, A. et al. (2004) Oncogene 23:7552. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Human IL-18/IL-1F4 Antibody

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