Human IL-15 Antibody

Name: Human IL-15 Antibody
Brand: R&D Systems
SKU: MAB247

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB247

Citations (12)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-15 Asn49-Ser162 Accession # P40933
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 34593
Specificity:	Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL‑2 is observed.

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Applications for Human IL-15 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑15 (Catalog # 247-ILB)

Item	Value
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 0.007-0.042 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-15 Antibody

	Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL-15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-15 Monoclonal Antibody (Catalog # MAB247). The ND₅₀ is typically 0.007-0.042 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8⁺ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

Item	Value
References	Grabstein, K. et al. (1994) Science 264:965. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444. Giri, J.G. et al. (1995) EMBO 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851. Dubois, S. et al. (2002) Immunity 17:537. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names	IL-15, IL-15MGC9721, IL15, interleukin 15, interleukin-15

Citations for Human IL-15 Antibody (12)

Showing 1 - 10 of 12 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-15 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

L Swadling et al. (2020), Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence Cell Rep, 30(3):687-698.e6.
PMID: 31968246
Species: Human
Sample Types: Extracellular Vesicles
Applications: Transmission Electron Microsco

DC Watson et al. (2018), Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes J Extracell Vesicles, 7(1):1442088.
PMID: 29535850
Species: Human
Sample Types: Protein
Applications: Neutralization

Nata T et al. (2015), Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 290(37):22338-51.
PMID: 26183780
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Benito-Miguel M et al. (2012), IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. PLoS ONE, 7(7):e40620.
PMID: 22792388
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Sato N et al. (2011), Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha. Blood, 117(15):4032-40.
PMID: 21304101
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Perrot I et al. (2010), TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA. J. Immunol., 185(4):2080-8.
PMID: 20639488
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Filer A et al. (2006), Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum., 54(7):2096-108.
PMID: 16802344
Species: Feline
Sample Types: Whole Cells
Applications: ICC

Dean GA et al. (2005), Cloning and expression of feline interleukin 15. Cytokine, 29(2):77-83.
PMID: 15598442
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ferlazzo G et al. (2004), Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs. Proc. Natl. Acad. Sci. U.S.A., 101(47):16606-11.
PMID: 15536127
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Miranda-Carus ME et al. (2004), IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. J. Immunol., 173(2):1463-76.
PMID: 15240743
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Briard D et al. (2002), Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 168(9):4326-32.
PMID: 11970974
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Corinti S et al. (2001), Regulatory activity of autocrine IL-10 on dendritic cell functions. J. Immunol., 166(7):4312-8.
PMID: 11254683

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