Human IgG Fc Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB110
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Control, ELISA Detection, ELISA Development, Neutralization, Neutralization Control, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 97924
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IgG Fc Antibody
Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
(A) Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). (B) In the absence of rhB7-H1/PD-L1 Fc Chimera, HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human IgG Fc Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP
Western Blot
1 µg/mL
Sample: Recombinant Human IgG Fc region under non-reducing conditions only
Sample: Recombinant Human IgG Fc region under non-reducing conditions only
Reviewed Applications
Read 3 reviews rated 4.7 using MAB110 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Human IgG Fc Antibody
Product Specific Notices for Human IgG Fc Antibody
For research use only
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