Human IgG Fc Antibody

Name: Human IgG Fc Antibody
Brand: R&D Systems
SKU: MAB110
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB110

Citations (6)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IgG₁ Fc region
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 97924
Specificity:	Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.

View Full Technical Details for Human IgG Fc Antibody

Applications for Human IgG Fc Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IgG Fc region under non-reducing conditions only
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IgG Fc Antibody

	Flow Cytometry Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. (A) Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). (B) In the absence of rhB7-H1/PD-L1 Fc Chimera, HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G
Alternate Names	IgG, Immunoglobulin G, ImmunoglobulinG

Citations for Human IgG Fc Antibody (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IgG Fc Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Y Yoon et al. (2020), PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival Mol. Psychiatry, 0(0).
PMID: 32541930
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development

M Yang et al. (2020), Tumour-associated neutrophils orchestrate intratumoural IL-8-driven immune evasion through Jagged2 activation in ovarian cancer Br. J. Cancer, 0(0).
PMID: 32778818
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2016), Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-? Aging Cell, 0(0).
PMID: 27660103
Species: Human
Sample Types: Whole Cells
Applications: Control

Wan-Chien Cheng (2016), Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Eur J Immunol, 0(0).
PMID: 27334899
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization Control

Kotani Y et al. (2014), Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement. PLoS ONE, 9(3):e91857.
PMID: 24632732
Species: Human
Sample Types: Serum
Applications: ELISA Development

(2012), Epithelial junction opener JO-1 improves monoclonal antibody therapy of cancer. Cancer Res., 71(22):7080-90.
PMID: 21990319

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Customer Reviews for Human IgG Fc Antibody (2)

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Human IgG Fc Antibody

Name: Anonymous

Application: Blocked membrane with 5% milk in TBS-T (0.1%) for 2h at RTUsed 500ng/ml antibody in 5% milk overnight at 4CUsed ECL system for detection

Sample Tested: Recombinant protein

Species: Human

Verified Customer | Posted 07/01/2022
Human IgG Fc Antibody

Name: Anonymous

Application: WB

Sample Tested: HEK293 Human Cell Line

Species: Human

Verified Customer | Posted 10/09/2021

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Product Documents for Human IgG Fc Antibody

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Human IgG Fc Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human IgG Fc Antibody

Scientific Data Examples for Human IgG Fc Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IgG

Citations for Human IgG Fc Antibody (6)

Customer Reviews for Human IgG Fc Antibody (2)

Customer Images

Human IgG Fc Antibody

Human IgG Fc Antibody

Product Documents for Human IgG Fc Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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