Human HVEM/TNFRSF14 APC-conjugated Antibody

Name: Human HVEM/TNFRSF14 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB356A

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB356A

Citations (3)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14 Pro37-Val202 (Ser108Thr and Ala140Arg) Accession # AAB58354
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 94801
Specificity:	Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD30 and no cross-reactivity with rh4-1BB, rhCD27, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhNGF-R, rhOPG, rhRANK, recombinant mouse (rm) RANK, rhTAJ, rhTNF RI, rhTNF RII, or rmTNF RI is observed.

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Applications for Human HVEM/TNFRSF14 APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human HVEM/TNFRSF14 APC-conjugated Antibody

	Flow Cytometry Detection of HVEM/TNFRSF14 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HVEM/TNFRSF14 APC-conjugated Monoclonal Antibody (Catalog # FAB356A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also known as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta (also known as lymphotoxin alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

Item	Value
References	Hsu, H. et al. (1997) J. Biol. Chem. 272:13471. Mauri, D.N. et al. (1998) Immunity 8:21. Montgomery, R.I. et al. (1996) Cell 87:427.
Long Name	Herpesvirus Entry Mediator
Entrez Gene IDs	8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names	ATAR, CD270, CD270 antigen, CD40-like protein, HVEM, HVEMTR2HVEAATAR, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA, LIGHTR, TNFRSF14, Tumor necrosis factor receptor-like 2, tumor necrosis factor receptor superfamily member 14, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator), tumor necrosis factor receptor-like gene2

Citations for Human HVEM/TNFRSF14 APC-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human HVEM/TNFRSF14 APC-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1. BMC Cancer, 12(0):176.
PMID: 22583667
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Wang C et al. (2007), 4-1BBL induces TNF receptor-associated factor 1-dependent Bim modulation in human T cells and is a critical component in the costimulation-dependent rescue of functionally impaired HIV-specific CD8 T cells. J. Immunol., 179(12):8252-63.
PMID: 18056369
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zhou J et al. (2005), Telomere length of transferred lymphocytes correlates with in vivo persistence and tumor regression in melanoma patients receiving cell transfer therapy. J. Immunol., 175(10):7046-52.
PMID: 16272366

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Human HVEM/TNFRSF14 APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human HVEM/TNFRSF14 APC-conjugated Antibody

Scientific Data Examples for Human HVEM/TNFRSF14 APC-conjugated Antibody

Flow Cytometry