Human HAI-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1048
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, Simple Western
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Glu449
Accession # NP_003701
Pro37-Glu449
Accession # NP_003701
Specificity
Detects human HAI-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouseHAI‑1 is observed and less than 1% cross-reactivity with recombinant human HAI-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human HAI-1 Antibody
Detection of Human HAI‑1 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HAI-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HAI‑1 in Human Lung Cancer Tissue.
HAI-1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human HAI‑1 by Simple WesternTM.
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for HAI-1 at approximately 88 kDa (as indicated) using 10 µg/mL of Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human HAI-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Simple Western
10 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-1
HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
References
- Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
- Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
- Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Alternate Names
HAI1, SPINT1
Gene Symbol
SPINT1
UniProt
Additional HAI-1 Products
Product Documents for Human HAI-1 Antibody
Product Specific Notices for Human HAI-1 Antibody
For research use only
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