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Human GFAP Antibody

Catalog # MAB25941 | R&D Systems, Inc. a Bio-Techne Brand
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MAB25941-100
MAB25941-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 987268

Product Summary for Human GFAP Antibody

Immunogen

Synthetic peptide containing human GFAP
Accession # P14136

Specificity

Detects human GFAP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GFAP Antibody

Detection of Human GFAP antibody by Western Blot.

Detection of Human GFAP by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue, human brain (motor cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GFAP antibody in U-251MG Human Cell Line by Immunocytochemistry (ICC).

GFAP in U-251MG Human Cell Line.

GFAP was detected in immersion fixed U-251MG human glioblastoma cell line using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GFAP antibody in Human Brain by Immunohistochemistry (IHC-P).

GFAP in Human Brain.

GFAP was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) at 0.2 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human GFAP Antibody

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed U-251MG human glioblastoma cell line

Immunohistochemistry

0.2-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)

Knockout Validated

GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line

Simple Western

10 µg/mL
Sample: Human cerebellum

Western Blot

0.25 µg/mL
Sample: human brain (cerebellum) tissue, human brain (motor cortex) tissue, and human brain (hypothalamus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is the predominant component of astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

GFAP, ALXDRD, FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

Product Documents for Human GFAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GFAP Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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