Human Galectin-4 Antibody

Name: Human Galectin-4 Antibody
Brand: R&D Systems
SKU: MAB1227
Rating: 5 (6 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1227

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Galectin-4 Ala2-Ile323 Accession # P56470
Applications:	Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 198616
Specificity:	Detects human Galectin-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -7, -8, recombinant mouse Galectin-1, -3, -4, or -7 is observed.

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Applications for Human Galectin-4 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Galectin-4 Antibody

	Western Blot Detection of Human Galectin‑4 by Western Blot. Western blot shows lysates of human colon tissue, COLO 205 human colorectal adenocarcinoma cell line, T84 human colon carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-4 Monoclonal Antibody (Catalog # MAB1227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-4

Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival and apoptosis, and are often up- or down-regulated in cancer (1‑3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on red cell blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7, 9). Human Galectin-4 shares 76%, 77%, 78% and 80% aa identity with mouse, rat, bovine and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues (5).

Item	Value
References	Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17. Elola, M. T. et al. (2007) Cell. Mol. Life Sci. 64:1679. Huflejt, M. E. & H. Leffler (2004) Glycoconj. J. 20:247. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081. Delacour, D. et al. (2005) J. Cell Biol. 169:491. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679. Stechly, L. et al. (2009) Traffic Jan 24 e-pub ahead of print. Entrez Accession # EAW56820. Hokama, A. et al. (2004) Immunity 20:681. Paclik, D. et al. (2008) PloS ONE 3:e2629.
Entrez Gene IDs	3960 (Human); 16855 (Mouse)
Alternate Names	Antigen NY-CO-27, GAL4, Galectin-4, Galectin4, L-36 lactose-binding protein, L36LBP, LGALS4, Lactose-binding lectin 4, gal-4, galectin 4, lectin, galactoside-binding, soluble, 4

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Customer Reviews for Human Galectin-4 Antibody (6)

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Human Galectin‑4 Antibody

Name: Anonymous

Application: WB

Sample Tested: Cell Lysates

Species: Human

Verified Customer | Posted 10/26/2015

AS seen, the antibody is very specific in detecting only galectin-4 and no other non-specific bands. Specificity: SpecificSensitivity: SensitiveBuffer: Milk, BSA, MilkDilution: 1:1000
Human Galectin‑4 Antibody

Name: Anonymous

Application: IP

Sample Tested: Cancer cell lysates

Species: Human

Verified Customer | Posted 10/26/2015

As seen, the antibody is specific and sensitive in detecting 36kDa of galectin-4, while 50 kDa is the antibody bands. Specificity: SpecificSensitivity: SensitiveBuffer: IP bufferDilution: 1:1000
Human Galectin‑4 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Colon tissue

Species: Human

Verified Customer | Posted 10/26/2015

The antibody specifically detects the crypt epithelial cells at lower dilutions also. Mouse antibody specific IHC kit was used for staining. Specificity: SpecificSensitivity: SensitiveBuffer: PBSDilution: 1:500
Human Galectin‑4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Cancer Cells

Species: Human

Verified Customer | Posted 10/26/2015

Serves very well to detect the protein using IFC. Specificity: SpecificSensitivity: SensitiveBuffer: PBSDilution: 1:1000
Human Galectin‑4 Antibody

Name: Anonymous

Application: Flow

Sample Tested: Cancer Cells

Species: Human

Verified Customer | Posted 10/26/2015

The antibody is very sensitive to detect internal galectin-4 using this antibody. Specificity: SpecificSensitivity: SensitiveBuffer: Fixing bufferDilution: 1:100
Human Galectin‑4 Antibody

Name: Anonymous

Application: 2D gel, protein modification

Sample Tested: Cell Lysates

Species: Human

Verified Customer | Posted 10/26/2015

2D gel was probed for galectin-4 and works very well. Specificity: Reasonably specificSensitivity: SensitiveBuffer: BSADilution: 1:1000

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Human Galectin-4 Antibody

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Applications for Human Galectin-4 Antibody

Scientific Data Examples for Human Galectin-4 Antibody

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-4

Citations for Human Galectin-4 Antibody

Customer Reviews for Human Galectin-4 Antibody (6)

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Human Galectin‑4 Antibody

Human Galectin‑4 Antibody

Human Galectin‑4 Antibody

Human Galectin‑4 Antibody

Human Galectin‑4 Antibody

Human Galectin‑4 Antibody

Product Documents for Human Galectin-4 Antibody

Product Datasheets

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