Human Galectin-1 Antibody

Name: Human Galectin-1 Antibody
Brand: R&D Systems
SKU: AF1152
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1152

Citations (14)

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Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Galectin‑1 Ala2-Asp135 Accession # P09382
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Galectin-1 in direct ELISAs and Western blots.

View Full Technical Details for Human Galectin-1 Antibody

Applications for Human Galectin-1 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Galectin-1 Antibody

	Western Blot Detection of Human Galectin‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Galectin-1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Galectin‑1 in Human Prostate Cancer Tissue. Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry Galectin‑1 in Human Prostate Cancer Tissue. Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of stromal cells and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Intracellular Staining by Flow Cytometry Detection of Galectin‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P) and either (A) Normal Goat IgG Control (AB-108-C) or (B) Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
	Simple Western Detection of Human Galectin‑1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-1 at approximately 17 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins.

Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity.

Item	Value
Entrez Gene IDs	3956 (Human); 16852 (Mouse)
Alternate Names	14 kDa laminin-binding protein, 14 kDa lectin, BHL, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, GAL1, GBP, Galaptin, Galectin-1, Galectin1, HBL, HLBP14, HPL, L-14, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, gal-1, galectin 1, lectin, galactoside-binding, soluble, 1

Citations for Human Galectin-1 Antibody (14)

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Citations are publications that use Bio-Techne products. Selected citations for Human Galectin-1 Antibody include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

HM Hsu et al. (2023), Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates PloS Neglected Tropical Diseases, 17(1):e0011016.
PMID: 36595499
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

R Roldán-Mon et al. (2022), Galectin-1 prevents pathological vascular remodeling in atherosclerosis and abdominal aortic aneurysm Science Advances, 8(11):eabm7322.
PMID: 35294231
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation

P Szodoray et al. (2021), Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity Cell Reports, 36(6):109525.
PMID: 34380042
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

I Hirose et al. (2019), Glucocorticoid receptor inhibits Mï¿½ller glial galectin-1 expression via DUSP1-dependent and -independent deactivation of AP-1 signalling J. Cell. Mol. Med., 0(0).
PMID: 31328390
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

H Paz et al. (2018), Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008 J. Exp. Clin. Cancer Res., 37(1):67.
PMID: 29580262
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture)

MN Andersen et al. (2017), Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation Onco Targets Ther, 10(0):1977-1982.
PMID: 28435287
Species: Human
Sample Types: Whole Tissue
Applications: IHC

H Schulz et al. (2017), Galectins-1, -3, and -7 Are Prognostic Markers for Survival of Ovarian Cancer Patients Int J Mol Sci, 18(6).
PMID: 28594391
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Prototype and Chimera-Type Galectins in Placentas with Spontaneous and Recurrent Miscarriages Int J Mol Sci, 17(5).
PMID: 27136536
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Yazawa E et al. (2015), Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. J Invest Dermatol, 135(7):1849-62.
PMID: 25756799
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Leskela S et al. (2015), Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells. PLoS ONE, 10(4):e0123938.
PMID: 25880730
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ryu K et al. (2014), Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury. Exp Cell Res, 326(1):143-54.
PMID: 24954408
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Suzuki O et al. (2012), Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells. Lab. Invest., 92(2):191-9.
PMID: 21986812
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Zhuo Y et al. (2008), Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis. J. Biol. Chem., 283(32):22177-85.
PMID: 18676377
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Chung L et al. (0), Galectin-1 promotes lung cancer progression and chemoresistance by upregulating p38 MAPK, ERK, and cyclooxygenase-2. Clin Cancer Re18(15):4037-47.
PMID: 22696230

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Customer Reviews for Human Galectin-1 Antibody (1)

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Human Galectin-1 Antibody

Name: Juliana Redondo

Application: WB

Sample Tested: Mesenchymal stem cells

Species: Human

Verified Customer | Posted 11/24/2016

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Product Documents for Human Galectin-1 Antibody

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Human Galectin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human Galectin-1 Antibody

Scientific Data Examples for Human Galectin-1 Antibody

Western Blot

Immunohistochemistry

Immunohistochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-1

Citations for Human Galectin-1 Antibody (14)

Customer Reviews for Human Galectin-1 Antibody (1)

Customer Images

Human Galectin-1 Antibody

Product Documents for Human Galectin-1 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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