Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 18 hours. PVDF Membrane was probed with 1 µg/mL of Human FRA-1 Monoclonal Antibody (Catalog # MAB4935) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FRA-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: A172 human glioblastoma cell line treated with Recombiant Human EGF (Catalog # 236-EG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRA-1

FRA-1 (FOSL1) is a 271 amino acid member of the leucine zipper Fos family of transcription factors. Fos family members have been associated with cellular proliferation, differentiation and transformation. In accordance with these findings, overexpression of FRA-1 correlates with a malignant phenotype in breast cancer. In lung epithelia cells, FRA-1 promotes motility, invasion, and anchorage independent growth. FRA-1 knock out mice rescued for the FRA-1 placental defect are viable but show reduced bone formation that may be due to a defect in the osteoblast differentiation. Human FRA-1 and mouse FRA-1 are 94% identical between amino acids 146-247.

Long Name

Fos-related antigen 1

Alternate Names

FOSL1, FRA1

Entrez Gene IDs

8061 (Human); 14283 (Mouse); 25445 (Rat)

Gene Symbol

FOSL1

UniProt

P15407

Additional FRA-1 Products

Product Documents for Human FRA-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FRA-1 Antibody

For research use only

Citations
Reviews

Human FRA-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4935

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all FRA-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FRA-1 Antibody

Detection of Human FRA‑1 by Western Blot.