Human FGFR3 (IIIc) Antibody

Name: Human FGFR3 (IIIc) Antibody
Brand: R&D Systems
SKU: MAB7662

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB7662

Citations (2)

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Clone 136312 was used by HLDA to establish CD designation

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Species Reactivity:	Human
Immunogen:	Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 136312
Specificity:	Detects human FGF R3 (IIIc) in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) FGF R2 (IIIc), recombinant mouse (rm) FGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. In Western blots (non-reducing conditions only), 50‑100% cross-reactivity with rhFGF R2 (IIIc), rmFGF R2 (IIIc) and rmFGF R3 (IIIc) is observed.

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Applications for Human FGFR3 (IIIc) Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR) under non-reducingconditions.
Immunohistochemistry	5-25 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human FGFR3 (IIIc) Antibody

	Flow Cytometry Detection of FGFR3 (IIIc) in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.
	Immunocytochemistry FGFR3 in HepG2 Human Cell Line. FGFR3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunocytochemistry FGFR3 in MCF‑7 Human Cell Line. FGFR3 was detected in immersion fixed MCF‑7 human breast cancer cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunohistochemistry FGF R3 in Human Kidney. FGF R3 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Immunocytochemistry FGF R3 in U937 Human Cell Line. FGF R3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FGFR3

FGF R3 is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co-factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9 and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Item	Value
Long Name	Fibroblast Growth Factor Receptor 3
Entrez Gene IDs	2261 (Human); 14184 (Mouse)
Alternate Names	CD333, CEK, EC 2.7.10, FGF R3, FGFR3, HSFGFR3EX, JTK4, JTK4thanatophoric dwarfism, fibroblast growth factor receptor 3

Citations for Human FGFR3 (IIIc) Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGFR3 (IIIc) Antibody include:

Species: Mouse
Sample Types: Serum
Applications: ELISA Capture

D Gonçalves et al. (2020), In vitro and in vivo characterization of Recifercept, a soluble fibroblast growth factor receptor 3, as treatment for achondroplasia PLoS ONE, 15(12):e0244368.
PMID: 33370388
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Tomlinson DC et al. (2005), Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines. Cancer Res., 65(22):10441-9.
PMID: 16288035

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Product Documents for Human FGFR3 (IIIc) Antibody

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Human FGFR3 (IIIc) Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human FGFR3 (IIIc) Antibody

Scientific Data Examples for Human FGFR3 (IIIc) Antibody

Flow Cytometry

Immunocytochemistry

Immunocytochemistry

Immunohistochemistry

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: FGFR3

Citations for Human FGFR3 (IIIc) Antibody (2)

Product Documents for Human FGFR3 (IIIc) Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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