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Human FGF‑9 Antibody

Catalog # AF-273-NA | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity



Immunohistochemistry, Western Blot



Antibody Source

Polyclonal Goat IgG

Product Summary for Human FGF‑9 Antibody


S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9
Accession # P31371


Detects human FGF-9 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF‑4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-8, rhFGF-10, rhFGF-17, rhFGF-18, and rhFGF-19 is observed.







Scientific Data Images for Human FGF‑9 Antibody

FGF‑9 antibody in Human Placenta by Immunohistochemistry (IHC-P).

FGF‑9 in Human Placenta.

FGF-9 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Goat Anti-Human FGF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-273-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human FGF‑9 Antibody

Recommended Usage


5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF‑9 (Catalog # 273-F9)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Antigen Affinity-purified


Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-9

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. All FGFs have two conserved cysteine residues and share 30-50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts, and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.


  1. Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
  2. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.

Long Name

Fibroblast Growth Factor 9

Alternate Names


Entrez Gene IDs

2254 (Human); 14180 (Mouse)

Gene Symbol



Product Documents for Human FGF‑9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF‑9 Antibody

For research use only

Citations for Human FGF‑9 Antibody (4)

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Customer Reviews for Human FGF‑9 Antibody (1)

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  • Name: Anonymous -
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23797050
    Species: Human
    Verified Customer | Posted 01/07/2015

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