Human FGF‑9 Antibody

Name: Human FGF‑9 Antibody
Brand: R&D Systems
SKU: AF-273-NA
Rating: 5 (1 reviews)

Catalog # AF-273-NA | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (4)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human FGF‑9 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9
Met1-Ser208
Accession # P31371

Specificity

Detects human FGF-9 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF‑4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-8, rhFGF-10, rhFGF-17, rhFGF-18, and rhFGF-19 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FGF‑9 Antibody

FGF‑9 in Human Placenta.

FGF-9 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Goat Anti-Human FGF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-273-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human FGF‑9 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF‑9 (Catalog # 273-F9)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-9

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. All FGFs have two conserved cysteine residues and share 30-50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts, and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.

References

Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.

Long Name

Fibroblast Growth Factor 9

Alternate Names

FGF9

Entrez Gene IDs

2254 (Human); 14180 (Mouse)

Gene Symbol

FGF9

UniProt

P31371

Product Documents for Human FGF‑9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human FGF‑9 Antibody

For research use only

Citations
Reviews

Citations for Human FGF‑9 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGF‑9 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

T Seitz et al. (2020-03-11), Fibroblast Growth Factor 9 is expressed by activated hepatic stellate cells and promotes progression of hepatocellular carcinoma Sci Rep, 2020-010(1):4546.
PMID: 32161315
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Yin H et al. (2015-07-16), Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases. J Biol Chem, 2015-0290(36):22127-42.
PMID: 26183774
Species: Human
Sample Types: Whole Cells,Whole Tissue
Applications: Neutralization,IHC-P

Teishima J et al. (2011-10-18), Relationship between the localization of fibroblast growth factor 9 in prostate cancer cells and postoperative recurrence. Prostate Cancer Prostatic Dis., 2011-115(1):8-14.
PMID: 22006051
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Frontini MJ et al. (2011-04-17), Fibroblast growth factor 9 delivery during angiogenesis produces durable, vasoresponsive microvessels wrapped by smooth muscle cells. Nat. Biotechnol., 2011-029(5):421-7.
PMID: 21499246

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