Human ESAM Antibody

Name: Human ESAM Antibody
Brand: R&D Systems
SKU: MAB4204
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB4204

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human ESAM Gln30-Ala247 Accession # Q96AP7
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 408519
Specificity:	Detects human ESAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed.

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Applications for Human ESAM Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human ESAM Antibody

	Flow Cytometry Detection of ESAM in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).
	Immunocytochemistry ESAM in HUVEC Human Cells. ESAM was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ESAM

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69%, and 67% aa identity with monkey, canine, mouse, and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets, and megakaryocytes and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

Item	Value
References	Hirata, K-I. et al. (2001) J. Biol. Chem. 276:16223. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.
Long Name	Endothelial Cell Adhesion Molecule
Entrez Gene IDs	90952 (Human); 69524 (Mouse)
Alternate Names	2310008D05Rik, ESAM, HUEL (C4orf1)-interacting protein, LP4791 protein, W117m, endothelial cell adhesion molecule, endothelial cell-selective adhesion molecule

Citations for Human ESAM Antibody

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Human ESAM Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: HUVEC human umbilical vein endothelial cells

Species: Human

Verified Customer | Posted 07/04/2022

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Product Documents for Human ESAM Antibody

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Human ESAM Antibody

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Applications for Human ESAM Antibody

Scientific Data Examples for Human ESAM Antibody

Flow Cytometry

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: ESAM

Citations for Human ESAM Antibody

Customer Reviews for Human ESAM Antibody (1)

Customer Images

Human ESAM Antibody

Product Documents for Human ESAM Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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