Does the human Erythropoietin (Epo) polyclonal antibody (Catalog # AB-286-NA) bind Epo if the receptor is not present?

Name: Human Erythropoietin/EPO Antibody
Brand: R&D Systems
SKU: AB-286-NA

AB-286-NA binds Epo if the receptor is not present.

Human Erythropoietin/EPO Antibody

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AB-286-NA

Citations (3)

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Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line (CHO)-derived recombinant human Erythropoietin/EPO
Applications:	Block/Neutralize, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Rabbit IgG
Specificity:	Detects human Erythropoietin/EPO in direct ELISAs and Western blots.

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Applications for Human Erythropoietin/EPO Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 286-EP)
Immunohistochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically <3 µg/mL in the presence of 0.2 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Erythropoietin/EPO Antibody

	Neutralization Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.2 units/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA). The ND₅₀ is typically <3 µg/mL.
	Immunohistochemistry Erythropoietin/EPO in Human Kidney. Erythropoietin/EPO was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 1 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of EPO is required for biological activities in vivo. Mature human EPO shares 75%‑84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells. Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.

Item	Value
Entrez Gene IDs	2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names	ECYT5, EP, EPO, Erythropoietin, MGC138142, MVCD2, epoetin

Citations for Human Erythropoietin/EPO Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Erythropoietin/EPO Antibody include:

Species: Human
Sample Types: Protein
Applications: ELISA Detection

T Susantad et al. (2021), Modified recombinant human erythropoietin with potentially reduced immunogenicity Scientific Reports, 11(1):1491.
PMID: 33452310
Species: Transgenic Hamster
Sample Types: Cell Lysates
Applications: Western Blot

A Costello et al. (2019), Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer Metab. Eng., 0(0).
PMID: 30615942
Species: Human
Sample Types: Whole Cells
Applications: ICC

Hwang DS et al. (2007), Practical recombinant hybrid mussel bioadhesive fp-151. Biomaterials, 28(24):3560-8.
PMID: 17507090

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FAQs for Human Erythropoietin/EPO Antibody

Does the human Erythropoietin (Epo) polyclonal antibody (Catalog # AB-286-NA) bind Epo if the receptor is not present?

AB-286-NA binds Epo if the receptor is not present.

Human Erythropoietin/EPO Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human Erythropoietin/EPO Antibody

Scientific Data Examples for Human Erythropoietin/EPO Antibody

Neutralization

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Erythropoietin/EPO

Citations for Human Erythropoietin/EPO Antibody (3)

FAQs for Human Erythropoietin/EPO Antibody

Product Documents for Human Erythropoietin/EPO Antibody

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