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Human E‑Selectin/CD62E Antibody

Catalog # BBA16 | R&D Systems, Inc. a Bio-Techne Brand
Clone BBIG-E4 was used by HLDA to establish CD designation
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BBA16

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E4

Product Summary for Human E‑Selectin/CD62E Antibody

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E‑Selectin/CD62E Antibody

Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry.

Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
E-Selectin/CD62E antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

E-Selectin/CD62E in HUVEC Human Cells.

E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF-a (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin/ CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
E‑Selectin/CD62E antibody in Human Brain by Immunohistochemistry (IHC-P).

E‑Selectin/CD62E in Human Brain.

E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human E‑Selectin/CD62E Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human umbilical cord endothelial cells (HUVECs) cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (Catalog # 210-TA)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010)

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (occipital cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.

Western Blot

1 µg/mL
Sample: Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only

Human E-Selectin/CD62E Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-10 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)
  • Standard: Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using BBA16 in the following applications:

Published Applications

Read 54 publications using BBA16 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

Product Documents for Human E‑Selectin/CD62E Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human E‑Selectin/CD62E Antibody

For research use only

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