Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human Dopa Decarboxylase/DDC Specificity by Using Knockout Cell Line.

Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Dopa Decarboxylase/DDC Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Dopa Decarboxylase/DDC Antibody

Application

Recommended Usage

Knockout Validated

Dopa Decarboxylase/DDC is specifically detected in Jurkat human acute T cell leukemia parental cell line but is not detectable in Dopa Decarboxylase/DDC knockout Jurkat cell line.

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopa Decarboxylase/DDC

Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).

References

Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.

Alternate Names

AADC, DDC

Entrez Gene IDs

1644 (Human)

Gene Symbol

DDC

UniProt

P20711

Additional Dopa Decarboxylase/DDC Products

Product Documents for Human Dopa Decarboxylase/DDC Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Dopa Decarboxylase/DDC Antibody

For research use only

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Human Dopa Decarboxylase/DDC Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3564

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all Dopa Decarboxylase/DDC Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Dopa Decarboxylase/DDC Antibody

Detection of Dopa Decarboxylase/DDC Mouse by Western Blot.