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Human DDR1 Antibody

Catalog # AF2396 | R&D Systems, Inc. a Bio-Techne Brand
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AF2396
AF2396-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human DDR1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DDR1
Asp21-Thr416
Accession # Q08345

Specificity

Detects human DDR1 in direct ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant human DDR2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DDR1 Antibody

Detection of Human DDR1 antibody by Western Blot.

Detection of Human DDR1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DDR1 at approximately 100 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DDR1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

DDR1 in Human Breast Cancer Tissue.

DDR1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human DDR1 antibody by Simple WesternTM.

Detection of Human DDR1 by Simple WesternTM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DDR1 at approximately 137 kDa (as indicated) using 10 µg/mL of Goat Anti-Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human DDR1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal skin, breast cancer tissue, and ovarian cancer tissue

Simple Western

10 µg/mL
Sample: MCF‑7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using AF2396 in the following applications:

Published Applications

Read 4 publications using AF2396 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR1

DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases. Mature human DDR2 consists of a 398 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic region with a tyrosine kinase domain. Within the ECD, human DDR1 shares 53% aa sequence identity with human DDR2 and 93% with mouse and rat DDR1. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers. Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region. Two additional kinase deficient splice forms are expressed in colon cancer. The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. Over-expression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not.

Long Name

Discoidin Domain Receptor 1

Alternate Names

CAK, CD167a, RTK6, TrkE

Entrez Gene IDs

780 (Human)

Gene Symbol

DDR1

UniProt

Product Documents for Human DDR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DDR1 Antibody

For research use only

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FAQs for Human DDR1 Antibody

Showing  1 - 1 of 1 FAQ Showing All
  • Q: I was wondering if you offer, or are planning on offering, any antibodies targeted to specific phosphorylation sites on the receptor tyrosine kinase DDR1.

    A: At this time we do not offer and DDR1 antibodies specific for certain phosphorylation states.

Showing  1 - 1 of 1 FAQ Showing All

Reconstitution Calculator

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