Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, and rhSULT4A1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Detection of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line.

Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

0.5 µg/mL
Sample: Human liver tissue

Neutralization

Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 2A1/SULT2A1 (8.33 µg/mL, Catalog # 5828-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 33 μM) to Trans-Dehydroandrosterone (DHEA, 133 µM). The Neutralization Dose (ND₅₀) is typically 35.9 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 2A1/SULT2A1

Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1.

Alternate Names

DHEA-ST, DHEAS, hSTa, ST2A3, SULT2A1

Entrez Gene IDs

6822 (Human); 20859 (Mouse); 24912 (Rat)

Gene Symbol

SULT2A1

UniProt

Q06520

Additional Cytosolic Sulfotransferase 2A1/SULT2A1 Products

Product Documents for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

For research use only

Citations
Reviews

Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5828

Key Product Details

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Product Specifications

Immunogen

Specificity